Associative growth of Enterobacter aerogenes, Proteus morganii, and Klebsiella pneumoniae with Pseudomonas putida and of K. pneumoniae with Pseudomonas fluorescens occurred in tuna fish infusion broth at 10°C and 20°C. Tests for presence of histamine, cadaverine, and putrescine were made after incubation of broths for various times from 4 h to 14 d. Amines were derivatized with dansyl chloride, selected by high-performance liquid chromatography and detected spectrophotometrically. Ratios of histamine: cadaverine and of histamine: putrescine ranged from 15: 1 to 20:1 and from 50:1 to 200: 1, respectively. These ratios were not affected appreciably by the presence of P. putida or P. fluorescencens in the cultures with the other test bacteria.
- Gram-negative bacteria
- Tuna fish broth
ASJC Scopus subject areas
- Ecology, Evolution, Behavior and Systematics
- Applied Microbiology and Biotechnology