Amine Production During Associative Growth of Gram-Negative Bacteria in Tuna Fish Infusion Broth

Elliot T. Ryser, Steve L. Taylor, Elmer H. Marth

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Associative growth of Enterobacter aerogenes, Proteus morganii, and Klebsiella pneumoniae with Pseudomonas putida and of K. pneumoniae with Pseudomonas fluorescens occurred in tuna fish infusion broth at 10°C and 20°C. Tests for presence of histamine, cadaverine, and putrescine were made after incubation of broths for various times from 4 h to 14 d. Amines were derivatized with dansyl chloride, selected by high-performance liquid chromatography and detected spectrophotometrically. Ratios of histamine: cadaverine and of histamine: putrescine ranged from 15: 1 to 20:1 and from 50:1 to 200: 1, respectively. These ratios were not affected appreciably by the presence of P. putida or P. fluorescencens in the cultures with the other test bacteria.

Original languageEnglish (US)
Pages (from-to)545-554
Number of pages10
JournalSystematic and Applied Microbiology
Volume5
Issue number4
DOIs
StatePublished - Jan 1 1984

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Keywords

  • Amines
  • Cadaverine
  • Enterobacter
  • Gram-negative bacteria
  • Histamine
  • Klebsiella
  • Proteus
  • Pseudomonas
  • Putrescine
  • Tuna fish broth

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

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