Amine Production During Associative Growth of Gram-Negative Bacteria in Tuna Fish Infusion Broth

Elliot T. Ryser, Steve L. Taylor, Elmer H. Marth

Research output: Contribution to journalArticle

5 Scopus citations


Associative growth of Enterobacter aerogenes, Proteus morganii, and Klebsiella pneumoniae with Pseudomonas putida and of K. pneumoniae with Pseudomonas fluorescens occurred in tuna fish infusion broth at 10°C and 20°C. Tests for presence of histamine, cadaverine, and putrescine were made after incubation of broths for various times from 4 h to 14 d. Amines were derivatized with dansyl chloride, selected by high-performance liquid chromatography and detected spectrophotometrically. Ratios of histamine: cadaverine and of histamine: putrescine ranged from 15: 1 to 20:1 and from 50:1 to 200: 1, respectively. These ratios were not affected appreciably by the presence of P. putida or P. fluorescencens in the cultures with the other test bacteria.

Original languageEnglish (US)
Pages (from-to)545-554
Number of pages10
JournalSystematic and Applied Microbiology
Issue number4
StatePublished - Jan 1 1984



  • Amines
  • Cadaverine
  • Enterobacter
  • Gram-negative bacteria
  • Histamine
  • Klebsiella
  • Proteus
  • Pseudomonas
  • Putrescine
  • Tuna fish broth

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Cite this