Abstract
There is a great interest in developing the only human cathelicidin into therapeutic molecules. The major antimicrobial region of human LL-37 corresponds to residues 17–32. The resultant peptide GF-17 shows a broad spectrum of antimicrobial activity against both Gram-positive and Gram-negative bacteria. By reducing the hydrophobic content, converting the broad-spectrum GF-17 to two narrow-spectrum peptides (GF-17d3 and KR-12) with activity against Gram-negative bacteria is successful. This study demonstrates that substitution of multiple basic amino acids by hydrophobic alanines makes a broad-spectrum peptide 17BIPHE2 (designed based on GF-17d3) active against Staphylococcal pathogens but not other bacteria tested. Taken together, the results reveal distinct charge and hydrophobic requirements for peptides to kill Gram-positive or Gram-negative bacteria. This finding is in line with the bioinformatics analysis of the peptides in the Antimicrobial Peptide Database (http://aps.unmc.edu/AP). In addition, a hot-spot arginine is identified and used to design merecidin with reduced toxicity to human cells. Merecidin protects wax moth larvae (Galleria mellonella) from the infection of methicillin-resistant Staphylococcus aureus USA300. These new selective peptides constitute interesting candidates for future development.
Original language | English (US) |
---|---|
Article number | 1700259 |
Journal | Advanced Biosystems |
Volume | 2 |
Issue number | 5 |
DOIs | |
State | Published - May 2018 |
Keywords
- antimicrobial peptides
- cathelicidin
- hydrophobic content
- peptide design
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
- General Biochemistry, Genetics and Molecular Biology