Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus

A. I. Glukhov; S. A. Gordeev; S. V. Vinogradov; V. I. Kiselev; V. M. Kramarov; O. I. Kiselev; E. S. Severin

doi:10.1016/0890-8508(90)90002-H

Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus

A. I. Glukhov, S. A. Gordeev, S. V. Vinogradov, V. I. Kiselev, V. M. Kramarov, O. I. Kiselev, E. S. Severin

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.

Original language	English (US)
Pages (from-to)	435-443
Number of pages	9
Journal	Molecular and Cellular Probes
Volume	4
Issue number	6
DOIs	https://doi.org/10.1016/0890-8508(90)90002-H
State	Published - Dec 1990
Externally published	Yes

Keywords

DNA polymerase
DNA probes for Epstein-Barr virus
polymerase chain reaction

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/0890-8508(90)90002-H

Cite this

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title = "Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus",

abstract = "Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.",

keywords = "DNA polymerase, DNA probes for Epstein-Barr virus, polymerase chain reaction",

author = "Glukhov, {A. I.} and Gordeev, {S. A.} and Vinogradov, {S. V.} and Kiselev, {V. I.} and Kramarov, {V. M.} and Kiselev, {O. I.} and Severin, {E. S.}",

year = "1990",

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doi = "10.1016/0890-8508(90)90002-H",

language = "English (US)",

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TY - JOUR

T1 - Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus

AU - Glukhov, A. I.

AU - Gordeev, S. A.

AU - Vinogradov, S. V.

AU - Kiselev, V. I.

AU - Kramarov, V. M.

AU - Kiselev, O. I.

AU - Severin, E. S.

PY - 1990/12

Y1 - 1990/12

N2 - Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.

AB - Using thermophilic DNA-polymerase from Thermus thermophilus we have amplified by polymerase chain reaction (PCR) specific DNA sequences of Epstein-Barr virus (EBV) and human immunodeficiency virus (HIV). DNA-polymerase from Thermus thermophilus (molecular mass of 80-86 kDa) differs in its physico-chemical properties from DNA-polymerase from Thermus aquaticus (molecular mass of 62-68 kDa). To amplify the specific EBV DNA sequence, oligonucleotide primers for the virus replicon region (oriP region) were used. As a result of amplification, a specific 405-bp DNA fragment was produced.

KW - DNA polymerase

KW - DNA probes for Epstein-Barr virus

KW - polymerase chain reaction

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Amplification of DNA sequences of Epstein-Barr and human immunodeficiency viruses using DNA-polymerase from Thermus thermophilus

Abstract

Keywords

ASJC Scopus subject areas

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