An effective chromatographic fingerprinting workflow based on comprehensive two-dimensional gas chromatography – Mass spectrometry to establish volatiles patterns discriminative of spoiled hazelnuts (Corylus avellana L.)

Federico Stilo, Erica Liberto, Nicola Spigolon, Giuseppe Genova, Ginevra Rosso, Mauro Fontana, Stephen E. Reichenbach, Carlo Bicchi, Chiara Cordero

Research output: Contribution to journalArticlepeer-review

Abstract

The volatile fraction of hazelnuts encrypts information about: cultivar/geographical origin, post-harvest treatments, oxidative stability and sensory quality. However, sensory features could be buried under other dominant chemical signatures posing challenges to an effective classification based on pleasant/unpleasant notes. Here a novel workflow that combines Untargeted and Targeted (UT) fingerprinting on comprehensive two-dimensional gas-chromatographic patterns is developed to discriminate spoiled hazelnuts from those of acceptable quality. By flash-profiling, six hazelnut classes are defined: Mould, Mould-rancid-solvent, Rancid, Rancid-stale, Rancid-solvent, and Uncoded KO. Chromatographic fingerprinting on composite 2D chromatograms from samples belonging to the same class (i.e., composite class-images) enabled effective selection of chemical markers: (a) octanoic acid that guides the sensory classification being positively correlated to mould; (b) ƴ-nonalactone, ƴ-hexalactone, acetone, and 1-nonanol that are decisive to classify OK and rancid samples; (c) heptanoic and hexanoic acids and ƴ-octalactone present in high relative abundance in rancid-solvent and rancid-stale samples.

Original languageEnglish (US)
Article number128135
JournalFood Chemistry
Volume340
DOIs
StatePublished - Mar 15 2021

Keywords

  • Combined Untargeted and Targeted (UT) fingerprinting
  • Comprehensive two-dimensional gas chromatography
  • Corylus avellana L.
  • Sensory defects
  • Supervised and unsupervised chemometrics
  • Visual features fingerprinting

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

Fingerprint Dive into the research topics of 'An effective chromatographic fingerprinting workflow based on comprehensive two-dimensional gas chromatography – Mass spectrometry to establish volatiles patterns discriminative of spoiled hazelnuts (Corylus avellana L.)'. Together they form a unique fingerprint.

Cite this