An enzyme-linked immunoassay for the cartilage proteoglycan

S. I. Rennard; K. Kimata; B. Dusemund; H. J. Barrach; J. Wilczek; J. H. Kimura; V. C. Hascall

doi:10.1016/0003-9861(81)90047-3

An enzyme-linked immunoassay for the cartilage proteoglycan

S. I. Rennard, K. Kimata, B. Dusemund, H. J. Barrach, J. Wilczek, J. H. Kimura, V. C. Hascall

Pulmonary, Critical Care, & Sleep Medicine

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Proteoglycan was purified from a rat chondrosarcoma and antiserum prepared. An enzyme-linked immunoassay was designed using this serum. The assay detected rat and murine, but not chick, high-molecular-weight cartilage proteoglycan. It did not detect noncartilage proteoglycan nor the low-molecular-weight proteoglycans found in cartilage. As little as 100 ng/ml of rat cartilage proteoglycan could be detected.

Original language	English (US)
Pages (from-to)	399-406
Number of pages	8
Journal	Archives of Biochemistry and Biophysics
Volume	207
Issue number	2
DOIs	https://doi.org/10.1016/0003-9861(81)90047-3
State	Published - Apr 1 1981

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

Access to Document

10.1016/0003-9861(81)90047-3

Fingerprint Dive into the research topics of 'An enzyme-linked immunoassay for the cartilage proteoglycan'. Together they form a unique fingerprint.

Cite this

@article{0026de28af6c4e44997b80d356bbcdde,

title = "An enzyme-linked immunoassay for the cartilage proteoglycan",

abstract = "Proteoglycan was purified from a rat chondrosarcoma and antiserum prepared. An enzyme-linked immunoassay was designed using this serum. The assay detected rat and murine, but not chick, high-molecular-weight cartilage proteoglycan. It did not detect noncartilage proteoglycan nor the low-molecular-weight proteoglycans found in cartilage. As little as 100 ng/ml of rat cartilage proteoglycan could be detected.",

author = "Rennard, {S. I.} and K. Kimata and B. Dusemund and Barrach, {H. J.} and J. Wilczek and Kimura, {J. H.} and Hascall, {V. C.}",

year = "1981",

month = apr,

day = "1",

doi = "10.1016/0003-9861(81)90047-3",

language = "English (US)",

volume = "207",

pages = "399--406",

journal = "Archives of Biochemistry and Biophysics",

issn = "0003-9861",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - An enzyme-linked immunoassay for the cartilage proteoglycan

AU - Rennard, S. I.

AU - Kimata, K.

AU - Dusemund, B.

AU - Barrach, H. J.

AU - Wilczek, J.

AU - Kimura, J. H.

AU - Hascall, V. C.

PY - 1981/4/1

Y1 - 1981/4/1

N2 - Proteoglycan was purified from a rat chondrosarcoma and antiserum prepared. An enzyme-linked immunoassay was designed using this serum. The assay detected rat and murine, but not chick, high-molecular-weight cartilage proteoglycan. It did not detect noncartilage proteoglycan nor the low-molecular-weight proteoglycans found in cartilage. As little as 100 ng/ml of rat cartilage proteoglycan could be detected.

AB - Proteoglycan was purified from a rat chondrosarcoma and antiserum prepared. An enzyme-linked immunoassay was designed using this serum. The assay detected rat and murine, but not chick, high-molecular-weight cartilage proteoglycan. It did not detect noncartilage proteoglycan nor the low-molecular-weight proteoglycans found in cartilage. As little as 100 ng/ml of rat cartilage proteoglycan could be detected.

UR - http://www.scopus.com/inward/record.url?scp=0019867620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019867620&partnerID=8YFLogxK

U2 - 10.1016/0003-9861(81)90047-3

DO - 10.1016/0003-9861(81)90047-3

M3 - Article

C2 - 7247411

AN - SCOPUS:0019867620

VL - 207

SP - 399

EP - 406

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 2

ER -