An enzyme-linked immunoassay for the cartilage proteoglycan

S. I. Rennard; K. Kimata; B. Dusemund; H. J. Barrach; J. Wilczek; J. H. Kimura; V. C. Hascall

doi:10.1016/0003-9861(81)90047-3

An enzyme-linked immunoassay for the cartilage proteoglycan

S. I. Rennard, K. Kimata, B. Dusemund, H. J. Barrach, J. Wilczek, J. H. Kimura, V. C. Hascall

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

Proteoglycan was purified from a rat chondrosarcoma and antiserum prepared. An enzyme-linked immunoassay was designed using this serum. The assay detected rat and murine, but not chick, high-molecular-weight cartilage proteoglycan. It did not detect noncartilage proteoglycan nor the low-molecular-weight proteoglycans found in cartilage. As little as 100 ng/ml of rat cartilage proteoglycan could be detected.

Original language	English (US)
Pages (from-to)	399-406
Number of pages	8
Journal	Archives of Biochemistry and Biophysics
Volume	207
Issue number	2
DOIs	https://doi.org/10.1016/0003-9861(81)90047-3
State	Published - Apr 1 1981
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology

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10.1016/0003-9861(81)90047-3

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author = "Rennard, {S. I.} and K. Kimata and B. Dusemund and Barrach, {H. J.} and J. Wilczek and Kimura, {J. H.} and Hascall, {V. C.}",

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AU - Rennard, S. I.

AU - Kimata, K.

AU - Dusemund, B.

AU - Barrach, H. J.

AU - Wilczek, J.

AU - Kimura, J. H.

AU - Hascall, V. C.

PY - 1981/4/1

Y1 - 1981/4/1

N2 - Proteoglycan was purified from a rat chondrosarcoma and antiserum prepared. An enzyme-linked immunoassay was designed using this serum. The assay detected rat and murine, but not chick, high-molecular-weight cartilage proteoglycan. It did not detect noncartilage proteoglycan nor the low-molecular-weight proteoglycans found in cartilage. As little as 100 ng/ml of rat cartilage proteoglycan could be detected.

AB - Proteoglycan was purified from a rat chondrosarcoma and antiserum prepared. An enzyme-linked immunoassay was designed using this serum. The assay detected rat and murine, but not chick, high-molecular-weight cartilage proteoglycan. It did not detect noncartilage proteoglycan nor the low-molecular-weight proteoglycans found in cartilage. As little as 100 ng/ml of rat cartilage proteoglycan could be detected.

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An enzyme-linked immunoassay for the cartilage proteoglycan

Abstract

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