An enzyme‐linked immunosorbent assay for the detection of antitetanus toxoid antibody using aluminum‐absorbed coating antigen

G. M. Thiele, J. Rogers, M. Collins, D. Smith, T. L. McDonald, N. Yasuda

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

An enzyme‐linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum‐absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum‐absorbed tetanus suspension. The specificity of the antibodies for tetanus antigen was tested both before and after antitetanus activity was removed with tetanus antigen–coated beads. Also, the activities of the sera were compared with those of a commercially available test. Our results indicated that the aluminum‐absorbed tetanus suspension can be coated onto standard polystyrene ELISA plates and used to measure antibody titers to tetanus toxoid.

Original languageEnglish (US)
Pages (from-to)126-129
Number of pages4
JournalJournal of Clinical Laboratory Analysis
Volume4
Issue number2
DOIs
StatePublished - 1990

Keywords

  • ELISA
  • Tetanus toxoid
  • aluminum‐absorbed coating
  • antibodies

ASJC Scopus subject areas

  • Immunology and Allergy
  • Hematology
  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology
  • Biochemistry, medical
  • Microbiology (medical)

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