An evolutionarily conserved protein fraction stably linked to DNA

Zoya Avramova; Ivailo Mikhailov; Roumen Tsanev

doi:10.1016/0167-4781(89)90137-1

An evolutionarily conserved protein fraction stably linked to DNA

Zoya Avramova, Ivailo Mikhailov, Roumen Tsanev

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Chromatins from four evolutionarily remote species (insect, fish, amphibian and bird) were isolated, high-salt-extracted and extensively deproteinized to remove noncovalently associated proteins. A protein fraction resisting the extraction procedures was found firmly linked to DNA in all four chromatins. Two-dimensional tryptic peptide mapping revealed a remarkable evolutionary conservativeness of this protein component, suggesting an indispensable function for it in the nucleus.

Original language	English (US)
Pages (from-to)	109-111
Number of pages	3
Journal	BBA - Gene Structure and Expression
Volume	1007
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(89)90137-1
State	Published - Jan 13 1989
Externally published	Yes

Keywords

Chromatin
DNA-protein interaction
Evolution

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(89)90137-1

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abstract = "Chromatins from four evolutionarily remote species (insect, fish, amphibian and bird) were isolated, high-salt-extracted and extensively deproteinized to remove noncovalently associated proteins. A protein fraction resisting the extraction procedures was found firmly linked to DNA in all four chromatins. Two-dimensional tryptic peptide mapping revealed a remarkable evolutionary conservativeness of this protein component, suggesting an indispensable function for it in the nucleus.",

keywords = "Chromatin, DNA-protein interaction, Evolution",

author = "Zoya Avramova and Ivailo Mikhailov and Roumen Tsanev",

year = "1989",

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doi = "10.1016/0167-4781(89)90137-1",

language = "English (US)",

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AU - Avramova, Zoya

AU - Mikhailov, Ivailo

AU - Tsanev, Roumen

PY - 1989/1/13

Y1 - 1989/1/13

N2 - Chromatins from four evolutionarily remote species (insect, fish, amphibian and bird) were isolated, high-salt-extracted and extensively deproteinized to remove noncovalently associated proteins. A protein fraction resisting the extraction procedures was found firmly linked to DNA in all four chromatins. Two-dimensional tryptic peptide mapping revealed a remarkable evolutionary conservativeness of this protein component, suggesting an indispensable function for it in the nucleus.

AB - Chromatins from four evolutionarily remote species (insect, fish, amphibian and bird) were isolated, high-salt-extracted and extensively deproteinized to remove noncovalently associated proteins. A protein fraction resisting the extraction procedures was found firmly linked to DNA in all four chromatins. Two-dimensional tryptic peptide mapping revealed a remarkable evolutionary conservativeness of this protein component, suggesting an indispensable function for it in the nucleus.

KW - Chromatin

KW - DNA-protein interaction

KW - Evolution

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DO - 10.1016/0167-4781(89)90137-1

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AN - SCOPUS:0024976807

SN - 0167-4781

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EP - 111

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1

ER -

An evolutionarily conserved protein fraction stably linked to DNA

Abstract

Keywords

ASJC Scopus subject areas

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