An expanded genetic code in mammalian cells with a functional quadruplet codon

Wei Niu, Peter G. Schultz, Jiantao Guo

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

We have utilized in vitro evolution to identify tRNA variants with significantly enhanced activity for the incorporation of unnatural amino acids into proteins in response to a quadruplet codon in both bacterial and mammalian cells. This approach will facilitate the creation of an optimized and standardized system for the genetic incorporation of unnatural amino acids using quadruplet codons, which will allow the biosynthesis of biopolymers that contain multiple unnatural building blocks.

Original languageEnglish (US)
Pages (from-to)1640-1645
Number of pages6
JournalACS chemical biology
Volume8
Issue number7
DOIs
StatePublished - Jul 19 2013

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine

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