An icosahedral algal virus has a complex unique vertex decorated by a spike

Mickaël V. Cherrier, Victor A. Kostyuchenko, Chuan Xiao, Valorie D. Bowman, Anthony J. Battisti, Xiaodong Yan, Paul R. Chipman, Timothy S. Baker, James L. Van Etten, Michael G. Rossmann

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Paramecium bursaria Chlorella virus-1 is an icosahedrally shaped, 1,900-Å-diameter virus that infects unicellular eukaryotic green algae. A 5-fold symmetric, 3D reconstruction using cryoelectron microscopy images has now shown that the quasiicosahedral virus has a unique vertex, with a pocket on the inside and a spike structure on the outside of the capsid. The pocket might contain enzymes for use in the initial stages of infection. The unique vertex consists of virally coded proteins, some of which have been identified. Comparison of shape, size, and location of the spike with similar features in bacteriophages T4 and P22 suggests that the spike might be a cell-puncturing device. Similar asymmetric features may have been missed in previous analyses of many other viruses that had been assumed to be perfectly icosahedral.

Original languageEnglish (US)
Pages (from-to)11085-11089
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number27
DOIs
StatePublished - Jul 7 2009

Keywords

  • 5-fold averaging
  • Cell entry
  • Cryoelectron microscopy reconstruction
  • Paramecium bursaria Chlorella virus-1
  • Specialized vertex protein

ASJC Scopus subject areas

  • General

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    Cherrier, M. V., Kostyuchenko, V. A., Xiao, C., Bowman, V. D., Battisti, A. J., Yan, X., Chipman, P. R., Baker, T. S., Van Etten, J. L., & Rossmann, M. G. (2009). An icosahedral algal virus has a complex unique vertex decorated by a spike. Proceedings of the National Academy of Sciences of the United States of America, 106(27), 11085-11089. https://doi.org/10.1073/pnas.0904716106