TY - JOUR
T1 - An improved method for the determination of galactosaminitol, glucosaminitol, glucosamine, and galactosamine on an amino acid analyzer
AU - Cheng, Pi wan
AU - Boat, Thomas F.
N1 - Funding Information:
’ This work was partially supported by Grant No. NO1 HRS-2957 from the U. S. Public Health Service and a Grant from the Health Fund for Cleveland.
PY - 1978/3
Y1 - 1978/3
N2 - Existing methods for the separation and quantitation of galactosaminitol, glucosaminitol, glucosamine, and galactosamine with an amino acid analyzer have been improved substantially by (1) addition of 15% ( v v) 1-propanol to the citrate-borate buffer, (2) utilization of a two-buffer system, (3) doubling the length of the ninhydrin reaction coil, and (4) inclusion of an internal standard, ε-amino-n-eaproic acid, in each sample. These modifications decrease the time for the separation of these compounds to 110 min and increase the sensitivity for hexosaminitols to 3-4 nmol, and that for hexosamines, to 2 nmol.
AB - Existing methods for the separation and quantitation of galactosaminitol, glucosaminitol, glucosamine, and galactosamine with an amino acid analyzer have been improved substantially by (1) addition of 15% ( v v) 1-propanol to the citrate-borate buffer, (2) utilization of a two-buffer system, (3) doubling the length of the ninhydrin reaction coil, and (4) inclusion of an internal standard, ε-amino-n-eaproic acid, in each sample. These modifications decrease the time for the separation of these compounds to 110 min and increase the sensitivity for hexosaminitols to 3-4 nmol, and that for hexosamines, to 2 nmol.
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U2 - 10.1016/0003-2697(78)90300-7
DO - 10.1016/0003-2697(78)90300-7
M3 - Article
C2 - 629386
AN - SCOPUS:0017840721
SN - 0003-2697
VL - 85
SP - 276
EP - 282
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -