An improved method for the determination of galactosaminitol, glucosaminitol, glucosamine, and galactosamine on an amino acid analyzer

Pi wan Cheng, Thomas F. Boat

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Existing methods for the separation and quantitation of galactosaminitol, glucosaminitol, glucosamine, and galactosamine with an amino acid analyzer have been improved substantially by (1) addition of 15% ( v v) 1-propanol to the citrate-borate buffer, (2) utilization of a two-buffer system, (3) doubling the length of the ninhydrin reaction coil, and (4) inclusion of an internal standard, ε-amino-n-eaproic acid, in each sample. These modifications decrease the time for the separation of these compounds to 110 min and increase the sensitivity for hexosaminitols to 3-4 nmol, and that for hexosamines, to 2 nmol.

Original languageEnglish (US)
Pages (from-to)276-282
Number of pages7
JournalAnalytical Biochemistry
Volume85
Issue number1
DOIs
StatePublished - Mar 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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