An improved miniprotein host for fluorogenic supramolecular assembly on the surface of living cells

Bi Xu; Xinqi Zhou; Cliff I. Stains

doi:10.1039/c6ra01215a

An improved miniprotein host for fluorogenic supramolecular assembly on the surface of living cells

Bi Xu, Xinqi Zhou, Cliff I. Stains

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2 Scopus citations

Abstract

An improved miniprotein host capable of fluorogenic supramolecular assembly with a sI-Pht guest is reported. This improved host, termed 6.2.22, displays significant enhancements in both the EC₅₀ for complexation and fluorescence activation of the sI-Pht guest, allowing for improved resolution of supramolecular complexation on the surface of living yeast.

Original language	English (US)
Pages (from-to)	20381-20385
Number of pages	5
Journal	RSC Advances
Volume	6
Issue number	24
DOIs	https://doi.org/10.1039/c6ra01215a
State	Published - 2016

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

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10.1039/c6ra01215a

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title = "An improved miniprotein host for fluorogenic supramolecular assembly on the surface of living cells",

abstract = "An improved miniprotein host capable of fluorogenic supramolecular assembly with a sI-Pht guest is reported. This improved host, termed 6.2.22, displays significant enhancements in both the EC50 for complexation and fluorescence activation of the sI-Pht guest, allowing for improved resolution of supramolecular complexation on the surface of living yeast.",

author = "Bi Xu and Xinqi Zhou and Stains, {Cliff I.}",

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year = "2016",

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AU - Xu, Bi

AU - Zhou, Xinqi

AU - Stains, Cliff I.

N1 - Publisher Copyright: © The Royal Society of Chemistry 2016.

PY - 2016

Y1 - 2016

N2 - An improved miniprotein host capable of fluorogenic supramolecular assembly with a sI-Pht guest is reported. This improved host, termed 6.2.22, displays significant enhancements in both the EC50 for complexation and fluorescence activation of the sI-Pht guest, allowing for improved resolution of supramolecular complexation on the surface of living yeast.

AB - An improved miniprotein host capable of fluorogenic supramolecular assembly with a sI-Pht guest is reported. This improved host, termed 6.2.22, displays significant enhancements in both the EC50 for complexation and fluorescence activation of the sI-Pht guest, allowing for improved resolution of supramolecular complexation on the surface of living yeast.

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U2 - 10.1039/c6ra01215a

DO - 10.1039/c6ra01215a

M3 - Article

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JO - RSC Advances

JF - RSC Advances

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ER -

An improved miniprotein host for fluorogenic supramolecular assembly on the surface of living cells

Abstract

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