An in vivo method for testing GABAergic compounds

Yiu K. Fung, A. Novin-Baheran, Teresa K. Bosh, Deanne R. Grassman

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

This report describes an intracerebroventricular technique of drug injection which enables compounds with GABAergic properties to be rapidly identified in vivo. In addition, this method allows for the testing of compounds that poorly penetrate the blood brain barrier for GABAergic activities. Subcutaneous administration of apomorphine (1.5 mg/kg, SC) elicits climbing behavior in mice. The apomorphine-induced climbing behavior is inhibited by the intracerebroventricular or intraperitoneal administration of known GABA (gamma aminobutyric acid) agonists including muscimol (10-50 ng) and GABA (2-10 μg). This inhibitory effect of GABA or muscimol on apomorphine-induced climbing behavior can be reversed by picrotoxin (2 mg/kg, IP), a known GABA-receptor antagonist.

Original languageEnglish (US)
Pages (from-to)651-654
Number of pages4
JournalPharmacology, Biochemistry and Behavior
Volume17
Issue number4
DOIs
StatePublished - Oct 1982

Keywords

  • Apomorphine
  • Climbing behavior
  • GABA
  • In vivo studies
  • Muscimol
  • Picrotoxin

ASJC Scopus subject areas

  • Biochemistry
  • Toxicology
  • Pharmacology
  • Clinical Biochemistry
  • Biological Psychiatry
  • Behavioral Neuroscience

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