TY - JOUR
T1 - An induced mass spawn of the hermaphroditic lion-paw scallop, Nodipecten subnodosus
T2 - Genetic assignment of maternal and paternal parentage
AU - Petersen, Jessica L.
AU - Ibarra, Ana M.
AU - Ramirez, José L.
AU - May, Bernie
N1 - Funding Information:
UCMEXUS—CONACYT (Collaborative Grant CN-04–61 to B.P.M. and A.M.I., Sabbatical Fellowship to A.M.I.); University of California Davis (Jastro-Shields Research Award, Humanities Award, and Hart-Cole-Goss Fellowship to J.L.P.).
PY - 2008
Y1 - 2008
N2 - The Pacific lion-paw scallop is commonly propagated for aquaculture by induced mass spawns of few individuals. Parentage of a mass spawn of this species has not been evaluated nor has the maternal and paternal contribution of each of these functional hermaphrodites to the progeny. Genotypes of 6 spawners and 374 resulting progeny at 6 microsatellite loci were coupled with mitochondrial DNA sequencing to assign maternal and paternal parentage. After the identification of a high proportion of null alleles (9.7%), microsatellite data revealed that 51.7% of the progenies were full siblings, with a significant, unequal contribution of the 6 spawners to the progeny. Three progenies were the result of self-fertilization. All spawners contributed paternally (though unequally); however, 2 spawners were the maternal parents of all but 7 progenies resulting in a variance effective population size of 3.52. DNA sequencing confirmed 4 microsatellite mutations within 4476 alleles scored, all in the paternal germ line. With minor exception, the loci conformed to Mendelian rules of segregation when null alleles were accounted for, and 2 loci were found to be linked. These results lend insight to the genetic composition of induced mass spawns and provide a basis for the development of more effective spawning techniques.
AB - The Pacific lion-paw scallop is commonly propagated for aquaculture by induced mass spawns of few individuals. Parentage of a mass spawn of this species has not been evaluated nor has the maternal and paternal contribution of each of these functional hermaphrodites to the progeny. Genotypes of 6 spawners and 374 resulting progeny at 6 microsatellite loci were coupled with mitochondrial DNA sequencing to assign maternal and paternal parentage. After the identification of a high proportion of null alleles (9.7%), microsatellite data revealed that 51.7% of the progenies were full siblings, with a significant, unequal contribution of the 6 spawners to the progeny. Three progenies were the result of self-fertilization. All spawners contributed paternally (though unequally); however, 2 spawners were the maternal parents of all but 7 progenies resulting in a variance effective population size of 3.52. DNA sequencing confirmed 4 microsatellite mutations within 4476 alleles scored, all in the paternal germ line. With minor exception, the loci conformed to Mendelian rules of segregation when null alleles were accounted for, and 2 loci were found to be linked. These results lend insight to the genetic composition of induced mass spawns and provide a basis for the development of more effective spawning techniques.
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U2 - 10.1093/jhered/esn012
DO - 10.1093/jhered/esn012
M3 - Article
C2 - 18334505
AN - SCOPUS:49949109219
SN - 0022-1503
VL - 99
SP - 337
EP - 348
JO - Journal of Heredity
JF - Journal of Heredity
IS - 4
ER -