TY - JOUR
T1 - An inexpensive high-throughput nuclear magnetic resonance tube cleaning apparatus
AU - Zhang, Bo
AU - Hodgson, James
AU - Hancock, Walter
AU - Powers, Robert
N1 - Funding Information:
This work was supported in part by the National Institute of Allergy and Infectious Diseases ( R21AI081154 and R01AI087668 ), the Nebraska Tobacco Settlement Biomedical Research Development Funds , and a Nebraska Research Council Interdisciplinary Research Grant to R.P. The research was performed in facilities renovated with support from the National Institutes of Health ( RR015468-01 ).
PY - 2011/9/15
Y1 - 2011/9/15
N2 - Large-scale nuclear magnetic resonance (NMR) tube cleaning is currently a bottleneck in high-throughput NMR ligand affinity screens. Expensive alternatives include discarding the NMR tubes after a single use (∼US $2-$8/tube), using commercial NMR tube cleaners (∼$15,000), and abandoning NMR tubes for flow probe technology (∼$75,000). Instead, we describe a relatively inexpensive (∼$400) and easily constructed apparatus that can clean 180 NMR tubes per hour while using a modest amount of solvent. The application of this apparatus significantly shortens the time to recycle NMR tubes while avoiding cross-contamination and tube damage.
AB - Large-scale nuclear magnetic resonance (NMR) tube cleaning is currently a bottleneck in high-throughput NMR ligand affinity screens. Expensive alternatives include discarding the NMR tubes after a single use (∼US $2-$8/tube), using commercial NMR tube cleaners (∼$15,000), and abandoning NMR tubes for flow probe technology (∼$75,000). Instead, we describe a relatively inexpensive (∼$400) and easily constructed apparatus that can clean 180 NMR tubes per hour while using a modest amount of solvent. The application of this apparatus significantly shortens the time to recycle NMR tubes while avoiding cross-contamination and tube damage.
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U2 - 10.1016/j.ab.2011.05.012
DO - 10.1016/j.ab.2011.05.012
M3 - Article
C2 - 21640698
AN - SCOPUS:80155199029
SN - 0003-2697
VL - 416
SP - 234
EP - 236
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -