An inositolphosphorylceramide synthase is involved in regulation of plant programmed cell death associated with defense in arabidopsis

Wenming Wang, Xiaohua Yang, Samantha Tangchaiburana, Roland Ndeh, Jonathan E. Markham, Yoseph Tsegaye, Teresa M. Dunn, Guo Liang Wang, Maria Bellizzi, James F. Parsons, Danielle Morrissey, Janis E. Bravo, Daniel V. Lynch, Shunyuan Xiao

Research output: Contribution to journalArticle

136 Scopus citations

Abstract

The Arabidopsis thaliana resistance gene RPW8 triggers the hypersensitive response (HR) to restrict powdery mildew infection via the salicylic acid-dependent signaling pathway. To further understand how RPW8 signaling is regulated, we have conducted a genetic screen to identify mutations enhancing RPW8-mediated HR-like cell death (designated erh). Here, we report the isolation and characterization of the Arabidopsis erh 1 mutant, in which the At2g37940 locus is knocked out by a T-DNA insertion. Loss of function of ERH1 results in salicylic acid accumulation, enhanced transcription of RPW8 and RPW8-dependent spontaneous HR-like cell death in leaf tissues, and reduction in plant stature. Sequence analysis suggests that ERH1 may encode the long-sought Arabidopsis functional homolog of yeast and protozoan inositolphosphorylceramide synthase (IPCS), which converts ceramide to inositolphosphorylceramide. Indeed, ERH1 is able to rescue the yeast aur1 mutant, which lacks the IPCS, and the erh1 mutant plants display reduced (∼53% of wild type) levels of leaf IPCS activity, indicating that ERH1 encodes a plant IPCS. Consistent with its biochemical function, the erh1 mutation causes ceramide accumulation in plants expressing RPW8. These data reinforce the concept that sphingolipid metabolism (specifically, ceramide accumulation) plays an important role in modulating plant programmed cell death associated with defense.

Original languageEnglish (US)
Pages (from-to)3163-3179
Number of pages17
JournalPlant Cell
Volume20
Issue number11
DOIs
StatePublished - Nov 2008

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Fingerprint Dive into the research topics of 'An inositolphosphorylceramide synthase is involved in regulation of plant programmed cell death associated with defense in arabidopsis'. Together they form a unique fingerprint.

  • Cite this

    Wang, W., Yang, X., Tangchaiburana, S., Ndeh, R., Markham, J. E., Tsegaye, Y., Dunn, T. M., Wang, G. L., Bellizzi, M., Parsons, J. F., Morrissey, D., Bravo, J. E., Lynch, D. V., & Xiao, S. (2008). An inositolphosphorylceramide synthase is involved in regulation of plant programmed cell death associated with defense in arabidopsis. Plant Cell, 20(11), 3163-3179. https://doi.org/10.1105/tpc.108.060053