A virus with morphological and physicochemical properties similar to those of the vertebrate caliciviruses was isolated from navel orangeworms, Amyelois transitella (Walker). Infected larvae contained two types of virus particles: a 185S, 38 nm cupped particle (ACSVi) with a single major polypeptide of 70 000 mol. wt. and a 165S, 28 nm smooth particle (ACSVii) with a single major polypeptide of 29 000 mol. wt. Larval frass contained a heterogeneous population of virus particles. Evidence is presented which suggests that the 38 nm particle degrades by proteolytic digestion to produce predominantly 28 nm particles in frass. Virus particles contained a single-stranded RNA of 36S (about 2.5 x 106 mol. wt.).
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