An RNA virus in Autographa californica nuclear polyhedrosis preparations: Detection and identification

T. J. Morris; P. V. Vail; Susan S. Collier

doi:10.1016/0022-2011(81)90123-3

An RNA virus in Autographa californica nuclear polyhedrosis preparations: Detection and identification

T. J. Morris, P. V. Vail, Susan S. Collier

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A 35-nm RNA virus of Trichoplusia ni (TRV) was detected in preparations of Autographa californica nuclear polyhedrosis virus (AcMNPV). In comparisons of methods of detection of TRV (gel electrophoresis, sucrose density gradient centrifugation, and serology), the enzyme-linked immunosorbent assay (ELISA) was the most sensitive method for quantitative detection of the contaminant virus in infected larvae and AcMNPV preparations. A scheme is proposed for the detection of contaminant viruses in NPVs being developed for biological control.

Original language	English (US)
Pages (from-to)	201-208
Number of pages	8
Journal	Journal of Invertebrate Pathology
Volume	38
Issue number	2
DOIs	https://doi.org/10.1016/0022-2011(81)90123-3
State	Published - Sep 1981
Externally published	Yes

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

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10.1016/0022-2011(81)90123-3

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title = "An RNA virus in Autographa californica nuclear polyhedrosis preparations: Detection and identification",

abstract = "A 35-nm RNA virus of Trichoplusia ni (TRV) was detected in preparations of Autographa californica nuclear polyhedrosis virus (AcMNPV). In comparisons of methods of detection of TRV (gel electrophoresis, sucrose density gradient centrifugation, and serology), the enzyme-linked immunosorbent assay (ELISA) was the most sensitive method for quantitative detection of the contaminant virus in infected larvae and AcMNPV preparations. A scheme is proposed for the detection of contaminant viruses in NPVs being developed for biological control.",

author = "Morris, {T. J.} and Vail, {P. V.} and Collier, {Susan S.}",

note = "Funding Information: t Mention of a trademark. proprietary product, or vendor does not constitute a guarantee or warranty by the USDA and does not imply its approval to the exclusion of other products or vendors that may also be suitable. ? This work was supported in part by a Berkeley Campus Biomedical Research Grant and by a USDA-SEA/FR cooperative agreement 5%9AH2-9-478 entitled “Determination of extraneous viral contaminants in Buculovirus preparations.”",

year = "1981",

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doi = "10.1016/0022-2011(81)90123-3",

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T1 - An RNA virus in Autographa californica nuclear polyhedrosis preparations

T2 - Detection and identification

AU - Morris, T. J.

AU - Vail, P. V.

AU - Collier, Susan S.

N1 - Funding Information: t Mention of a trademark. proprietary product, or vendor does not constitute a guarantee or warranty by the USDA and does not imply its approval to the exclusion of other products or vendors that may also be suitable. ? This work was supported in part by a Berkeley Campus Biomedical Research Grant and by a USDA-SEA/FR cooperative agreement 5%9AH2-9-478 entitled “Determination of extraneous viral contaminants in Buculovirus preparations.”

PY - 1981/9

Y1 - 1981/9

N2 - A 35-nm RNA virus of Trichoplusia ni (TRV) was detected in preparations of Autographa californica nuclear polyhedrosis virus (AcMNPV). In comparisons of methods of detection of TRV (gel electrophoresis, sucrose density gradient centrifugation, and serology), the enzyme-linked immunosorbent assay (ELISA) was the most sensitive method for quantitative detection of the contaminant virus in infected larvae and AcMNPV preparations. A scheme is proposed for the detection of contaminant viruses in NPVs being developed for biological control.

AB - A 35-nm RNA virus of Trichoplusia ni (TRV) was detected in preparations of Autographa californica nuclear polyhedrosis virus (AcMNPV). In comparisons of methods of detection of TRV (gel electrophoresis, sucrose density gradient centrifugation, and serology), the enzyme-linked immunosorbent assay (ELISA) was the most sensitive method for quantitative detection of the contaminant virus in infected larvae and AcMNPV preparations. A scheme is proposed for the detection of contaminant viruses in NPVs being developed for biological control.

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An RNA virus in Autographa californica nuclear polyhedrosis preparations: Detection and identification

Abstract

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