An RNA virus in Autographa californica nuclear polyhedrosis preparations: Detection and identification

T. J. Morris, P. V. Vail, Susan S. Collier

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A 35-nm RNA virus of Trichoplusia ni (TRV) was detected in preparations of Autographa californica nuclear polyhedrosis virus (AcMNPV). In comparisons of methods of detection of TRV (gel electrophoresis, sucrose density gradient centrifugation, and serology), the enzyme-linked immunosorbent assay (ELISA) was the most sensitive method for quantitative detection of the contaminant virus in infected larvae and AcMNPV preparations. A scheme is proposed for the detection of contaminant viruses in NPVs being developed for biological control.

Original languageEnglish (US)
Pages (from-to)201-208
Number of pages8
JournalJournal of Invertebrate Pathology
Volume38
Issue number2
DOIs
StatePublished - Sep 1981

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

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