An RNA virus in Autographa californica nuclear polyhedrosis virus preparations: Gross pathology and infectivity

P. V. Vail; T. J. Morris; S. S. Collier

doi:10.1016/0022-2011(83)90217-3

An RNA virus in Autographa californica nuclear polyhedrosis virus preparations: Gross pathology and infectivity

P. V. Vail, T. J. Morris, S. S. Collier

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The pathology and infectivity of an RNA virus infectious to Trichoplusia ni larvae was investigated. The enzyme-linked immunosorbent assay (ELISA) and weight depression were used as criteria for virus concentration in larval homogenates and live larvae, respectively. Infected larvae were severely stunted, weighing as little as 13 times less than uninfected individuals of the same age, yet appeared normal morphologically. The virus was found to cause only slight mortality at high concentrations. Infected larvae displayed the pathological stunting response down to a dose of 0.1 ng of virus. Larvae infected with doses 100 times lower did not show the weight response but such inapparent infections were detectable by ELISA. Because of these subtle gross pathological symptoms, particularly at low levels of infection, infected individuals could easily remain unde-tected in a group-reared colony.

Original language	English (US)
Pages (from-to)	179-183
Number of pages	5
Journal	Journal of Invertebrate Pathology
Volume	41
Issue number	2
DOIs	https://doi.org/10.1016/0022-2011(83)90217-3
State	Published - Mar 1983
Externally published	Yes

Keywords

Autographa californica
RNA virus
Trichoplusia ni
enzyme-linked immunosorbent assay (ELISA)
infectivity
nuclear polyhedrosis virus
pathology

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

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10.1016/0022-2011(83)90217-3

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title = "An RNA virus in Autographa californica nuclear polyhedrosis virus preparations: Gross pathology and infectivity",

abstract = "The pathology and infectivity of an RNA virus infectious to Trichoplusia ni larvae was investigated. The enzyme-linked immunosorbent assay (ELISA) and weight depression were used as criteria for virus concentration in larval homogenates and live larvae, respectively. Infected larvae were severely stunted, weighing as little as 13 times less than uninfected individuals of the same age, yet appeared normal morphologically. The virus was found to cause only slight mortality at high concentrations. Infected larvae displayed the pathological stunting response down to a dose of 0.1 ng of virus. Larvae infected with doses 100 times lower did not show the weight response but such inapparent infections were detectable by ELISA. Because of these subtle gross pathological symptoms, particularly at low levels of infection, infected individuals could easily remain unde-tected in a group-reared colony.",

keywords = "Autographa californica, RNA virus, Trichoplusia ni, enzyme-linked immunosorbent assay (ELISA), infectivity, nuclear polyhedrosis virus, pathology",

author = "Vail, {P. V.} and Morris, {T. J.} and Collier, {S. S.}",

note = "Funding Information: 2 Portions of the present study were supported by a USDA-AR-SEA Cooperative Agreement 58-9AHZ-9-478 entitled “Determination of extraneous viral contaminants in Baculovirus preparations,” with the Department of Plant Pathology, University of California, 333 Hilgard Hall, Berkeley, Calif.",

year = "1983",

month = mar,

doi = "10.1016/0022-2011(83)90217-3",

language = "English (US)",

volume = "41",

pages = "179--183",

journal = "Journal of Invertebrate Pathology",

issn = "0022-2011",

publisher = "Academic Press Inc.",

number = "2",

}

TY - JOUR

T1 - An RNA virus in Autographa californica nuclear polyhedrosis virus preparations

T2 - Gross pathology and infectivity

AU - Vail, P. V.

AU - Morris, T. J.

AU - Collier, S. S.

N1 - Funding Information: 2 Portions of the present study were supported by a USDA-AR-SEA Cooperative Agreement 58-9AHZ-9-478 entitled “Determination of extraneous viral contaminants in Baculovirus preparations,” with the Department of Plant Pathology, University of California, 333 Hilgard Hall, Berkeley, Calif.

PY - 1983/3

Y1 - 1983/3

N2 - The pathology and infectivity of an RNA virus infectious to Trichoplusia ni larvae was investigated. The enzyme-linked immunosorbent assay (ELISA) and weight depression were used as criteria for virus concentration in larval homogenates and live larvae, respectively. Infected larvae were severely stunted, weighing as little as 13 times less than uninfected individuals of the same age, yet appeared normal morphologically. The virus was found to cause only slight mortality at high concentrations. Infected larvae displayed the pathological stunting response down to a dose of 0.1 ng of virus. Larvae infected with doses 100 times lower did not show the weight response but such inapparent infections were detectable by ELISA. Because of these subtle gross pathological symptoms, particularly at low levels of infection, infected individuals could easily remain unde-tected in a group-reared colony.

AB - The pathology and infectivity of an RNA virus infectious to Trichoplusia ni larvae was investigated. The enzyme-linked immunosorbent assay (ELISA) and weight depression were used as criteria for virus concentration in larval homogenates and live larvae, respectively. Infected larvae were severely stunted, weighing as little as 13 times less than uninfected individuals of the same age, yet appeared normal morphologically. The virus was found to cause only slight mortality at high concentrations. Infected larvae displayed the pathological stunting response down to a dose of 0.1 ng of virus. Larvae infected with doses 100 times lower did not show the weight response but such inapparent infections were detectable by ELISA. Because of these subtle gross pathological symptoms, particularly at low levels of infection, infected individuals could easily remain unde-tected in a group-reared colony.

KW - Autographa californica

KW - RNA virus

KW - Trichoplusia ni

KW - enzyme-linked immunosorbent assay (ELISA)

KW - infectivity

KW - nuclear polyhedrosis virus

KW - pathology

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DO - 10.1016/0022-2011(83)90217-3

M3 - Article

AN - SCOPUS:0006297455

SN - 0022-2011

VL - 41

SP - 179

EP - 183

JO - Journal of Invertebrate Pathology

JF - Journal of Invertebrate Pathology

IS - 2

ER -

An RNA virus in Autographa californica nuclear polyhedrosis virus preparations: Gross pathology and infectivity

Abstract

Keywords

ASJC Scopus subject areas

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