An RNA virus in Autographa californica nuclear polyhedrosis virus preparations: Incidence and influence on baculovirus activity

P. V. Vail, T. J. Morris, S. S. Collier, B. Mackey

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

A small RNA virus infectious to Trichoplusia ni larvae (TRV) was observed as a contaminant of several Autographa californica nuclear polyhedrosis virus preparations (AcMNPV). The extent of contamination in various AcMNPV preparations was studied by means of serial enrichment passages through T. ni larvae and enzyme-linked immunosorbent assay (ELISA). TRV could not be detected by ELISA in the original preparation of AcMNPV polyhedra prepared in 1968 even after five enrichment passages. Antibody inactivation offers a possible prophylactic method against TRV but temperature inactivation (55°C) does not. Although TRV reduced larval weight, it had little or no effect on bioassays of AcMNPV to T. ni and Heliothis virescens.

Original languageEnglish (US)
Pages (from-to)171-178
Number of pages8
JournalJournal of Invertebrate Pathology
Volume41
Issue number2
DOIs
StatePublished - Mar 1983

Keywords

  • Autographa californica
  • Heliothis virescens
  • RNA virus
  • Spodoptera exigua
  • Trichoplusia ni
  • enzyme-linked immunosorbent assay (ELISA)
  • infectivity
  • nuclear polyhedrosis virus

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

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