An RNA virus in Autographa californica nuclear polyhedrosis virus preparations: Incidence and influence on baculovirus activity

P. V. Vail; T. J. Morris; S. S. Collier; B. Mackey

doi:10.1016/0022-2011(83)90216-1

An RNA virus in Autographa californica nuclear polyhedrosis virus preparations: Incidence and influence on baculovirus activity

P. V. Vail, T. J. Morris, S. S. Collier, B. Mackey

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A small RNA virus infectious to Trichoplusia ni larvae (TRV) was observed as a contaminant of several Autographa californica nuclear polyhedrosis virus preparations (AcMNPV). The extent of contamination in various AcMNPV preparations was studied by means of serial enrichment passages through T. ni larvae and enzyme-linked immunosorbent assay (ELISA). TRV could not be detected by ELISA in the original preparation of AcMNPV polyhedra prepared in 1968 even after five enrichment passages. Antibody inactivation offers a possible prophylactic method against TRV but temperature inactivation (55°C) does not. Although TRV reduced larval weight, it had little or no effect on bioassays of AcMNPV to T. ni and Heliothis virescens.

Original language	English (US)
Pages (from-to)	171-178
Number of pages	8
Journal	Journal of Invertebrate Pathology
Volume	41
Issue number	2
DOIs	https://doi.org/10.1016/0022-2011(83)90216-1
State	Published - Mar 1983
Externally published	Yes

Keywords

Autographa californica
Heliothis virescens
RNA virus
Spodoptera exigua
Trichoplusia ni
enzyme-linked immunosorbent assay (ELISA)
infectivity
nuclear polyhedrosis virus

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

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10.1016/0022-2011(83)90216-1

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title = "An RNA virus in Autographa californica nuclear polyhedrosis virus preparations: Incidence and influence on baculovirus activity",

abstract = "A small RNA virus infectious to Trichoplusia ni larvae (TRV) was observed as a contaminant of several Autographa californica nuclear polyhedrosis virus preparations (AcMNPV). The extent of contamination in various AcMNPV preparations was studied by means of serial enrichment passages through T. ni larvae and enzyme-linked immunosorbent assay (ELISA). TRV could not be detected by ELISA in the original preparation of AcMNPV polyhedra prepared in 1968 even after five enrichment passages. Antibody inactivation offers a possible prophylactic method against TRV but temperature inactivation (55°C) does not. Although TRV reduced larval weight, it had little or no effect on bioassays of AcMNPV to T. ni and Heliothis virescens.",

keywords = "Autographa californica, Heliothis virescens, RNA virus, Spodoptera exigua, Trichoplusia ni, enzyme-linked immunosorbent assay (ELISA), infectivity, nuclear polyhedrosis virus",

author = "Vail, {P. V.} and Morris, {T. J.} and Collier, {S. S.} and B. Mackey",

note = "Funding Information: I Mention of a proprietary product in this paper does not constitute an endorsement of this product by the U.S. Department of Agriculture. * Portions of the present study were supported by a USDA-ARS Cooperative Agreement 58-9AH2-9478 entitled “Determination of extraneous viral contaminants in Baculovirus preparations,” with the Department of Plant Pathology, University of California, 333 Hilgard Hall, Berkeley, Calif.",

year = "1983",

month = mar,

doi = "10.1016/0022-2011(83)90216-1",

language = "English (US)",

volume = "41",

pages = "171--178",

journal = "Journal of Invertebrate Pathology",

issn = "0022-2011",

publisher = "Academic Press Inc.",

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T1 - An RNA virus in Autographa californica nuclear polyhedrosis virus preparations

T2 - Incidence and influence on baculovirus activity

AU - Vail, P. V.

AU - Morris, T. J.

AU - Collier, S. S.

AU - Mackey, B.

N1 - Funding Information: I Mention of a proprietary product in this paper does not constitute an endorsement of this product by the U.S. Department of Agriculture. * Portions of the present study were supported by a USDA-ARS Cooperative Agreement 58-9AH2-9478 entitled “Determination of extraneous viral contaminants in Baculovirus preparations,” with the Department of Plant Pathology, University of California, 333 Hilgard Hall, Berkeley, Calif.

PY - 1983/3

Y1 - 1983/3

N2 - A small RNA virus infectious to Trichoplusia ni larvae (TRV) was observed as a contaminant of several Autographa californica nuclear polyhedrosis virus preparations (AcMNPV). The extent of contamination in various AcMNPV preparations was studied by means of serial enrichment passages through T. ni larvae and enzyme-linked immunosorbent assay (ELISA). TRV could not be detected by ELISA in the original preparation of AcMNPV polyhedra prepared in 1968 even after five enrichment passages. Antibody inactivation offers a possible prophylactic method against TRV but temperature inactivation (55°C) does not. Although TRV reduced larval weight, it had little or no effect on bioassays of AcMNPV to T. ni and Heliothis virescens.

AB - A small RNA virus infectious to Trichoplusia ni larvae (TRV) was observed as a contaminant of several Autographa californica nuclear polyhedrosis virus preparations (AcMNPV). The extent of contamination in various AcMNPV preparations was studied by means of serial enrichment passages through T. ni larvae and enzyme-linked immunosorbent assay (ELISA). TRV could not be detected by ELISA in the original preparation of AcMNPV polyhedra prepared in 1968 even after five enrichment passages. Antibody inactivation offers a possible prophylactic method against TRV but temperature inactivation (55°C) does not. Although TRV reduced larval weight, it had little or no effect on bioassays of AcMNPV to T. ni and Heliothis virescens.

KW - Autographa californica

KW - Heliothis virescens

KW - RNA virus

KW - Spodoptera exigua

KW - Trichoplusia ni

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KW - infectivity

KW - nuclear polyhedrosis virus

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ER -

An RNA virus in Autographa californica nuclear polyhedrosis virus preparations: Incidence and influence on baculovirus activity

Abstract

Keywords

ASJC Scopus subject areas

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