TY - JOUR
T1 - Anaerobic expression of maize fructose-1,6-diphosphate aldolase.
AU - Kelley, P. M.
AU - Freeling, M.
N1 - Copyright:
Medline is the source for the citation and abstract of this record.
PY - 1984/11/25
Y1 - 1984/11/25
N2 - The anaerobic proteins of maize are a set of 10 major and 10 minor polypeptides selectively synthesized in anaerobic seedling roots. 1) Anaerobiosis resulted in the selected labeling of a protein which bound to Blue Sepharose and was eluted by fructose 1,6-diphosphate. 2) This protein elicited antiserum which recognized a single protein with molecular weight of approximately 40,000. 3) By Western blot analysis, this antiserum recognized a maize fructose-1,6-diphosphate aldolase purified to homogeneity. We show that two major anaerobic proteins of maize, ANP35.5 and ANP33A, correspond to a cytoplasmic fructose-1,6-diphosphate aldolase.
AB - The anaerobic proteins of maize are a set of 10 major and 10 minor polypeptides selectively synthesized in anaerobic seedling roots. 1) Anaerobiosis resulted in the selected labeling of a protein which bound to Blue Sepharose and was eluted by fructose 1,6-diphosphate. 2) This protein elicited antiserum which recognized a single protein with molecular weight of approximately 40,000. 3) By Western blot analysis, this antiserum recognized a maize fructose-1,6-diphosphate aldolase purified to homogeneity. We show that two major anaerobic proteins of maize, ANP35.5 and ANP33A, correspond to a cytoplasmic fructose-1,6-diphosphate aldolase.
UR - http://www.scopus.com/inward/record.url?scp=0021771013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021771013&partnerID=8YFLogxK
M3 - Article
C2 - 6501292
AN - SCOPUS:0021771013
SN - 0021-9258
VL - 259
SP - 14180
EP - 14183
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 22
ER -