Anaerobic expression of maize fructose-1,6-diphosphate aldolase.

P. M. Kelley, M. Freeling

    Research output: Contribution to journalArticlepeer-review

    65 Scopus citations

    Abstract

    The anaerobic proteins of maize are a set of 10 major and 10 minor polypeptides selectively synthesized in anaerobic seedling roots. 1) Anaerobiosis resulted in the selected labeling of a protein which bound to Blue Sepharose and was eluted by fructose 1,6-diphosphate. 2) This protein elicited antiserum which recognized a single protein with molecular weight of approximately 40,000. 3) By Western blot analysis, this antiserum recognized a maize fructose-1,6-diphosphate aldolase purified to homogeneity. We show that two major anaerobic proteins of maize, ANP35.5 and ANP33A, correspond to a cytoplasmic fructose-1,6-diphosphate aldolase.

    Original languageEnglish (US)
    Pages (from-to)14180-14183
    Number of pages4
    JournalJournal of Biological Chemistry
    Volume259
    Issue number22
    StatePublished - Nov 25 1984

    ASJC Scopus subject areas

    • Biochemistry
    • Molecular Biology
    • Cell Biology

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