Analysis of 45 kb of DNA located at the left end of the chlorella virus PBCV-1 genorne

Zhiqiang Lu, Yu Li, Yanping Zhang, Gerald F. Kutish, Daniel L. Rock, James L. Van Etten

Research output: Contribution to journalArticlepeer-review

63 Scopus citations


Forty-five kilobases of DNA, including the previously sequenced 2.2-kb inverted repeat region, located at the left terminiof the 330-kb Chlorella virus PBCV-1 genome were sequenced and analyzed. Eighty-five complete open reading frames (ORFs) larger than 195 nucleotides were identified. Thirty-seven of the 85 ORFs, which are densely packed on both strands of the DNA, were considered major ORFs. Fifteen of the major ORFs have similarity to genes in the databases, including bacterial glycerophosphoryl diester phosphodiesterase, bacteriophage T4 endonuclease V, d-isomer specific 2-hydroxyacid dehydrogenases, and β-alanine synthetase and bacterial nitrilases. Two major ORFs resemble the virus major capsid protein. Three major ORFs contain three or more ankyrin-like repeat elements and four ORFs encode proline-rich proteins.

Original languageEnglish (US)
Pages (from-to)339-352
Number of pages14
Issue number1
StatePublished - Jan 10 1995
Externally publishedYes

ASJC Scopus subject areas

  • Virology


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