TY - JOUR
T1 - Analysis of 74 kb of DNA located at the right end of the 330-kb chlorella virus PBCV-1 genome
AU - Li, Yu
AU - Lu, Zhiqiang
AU - Sun, Liangwu
AU - Ropp, Susan
AU - Kutish, Gerald F.
AU - Rock, Daniel L.
AU - Van Etten, James L.
N1 - Funding Information:
We thank Xiaofen Liao, Bonnie Skuba, and Dwight Burbank for technical help and Les Lane and Yanping Zhang for helpful discussions. Chad Price kindly provided assistance with the computer analysis of the sequence. This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medical Sciences to J.V.E. Y.L. was supported in part by a University of Nebraska-Lincoln Center of Biotechnology Graduate Research Fellowship.
PY - 1997/10/27
Y1 - 1997/10/27
N2 - This report completes a preliminary analysis of the sequence of the 330,740-bp chlorella virus PBCV-1 genome, the largest virus genome to be sequenced to date. The PBCV-1 genome is 57% the size of the genome from the smallest self-replicating organism, Mycoplasma genitalium. Analysis of 74 kb of newly sequenced DNA, from the right terminus of the PBCV-1 genome, revealed 153 open reading frames (ORFs) of 65 codons or longer. Eighty-five of these ORFs, which are evenly distributed on both strands of the DNA, were considered major ORFs. Fifty-nine of the major ORFs were separated by less than 100 bp. The largest intergenic distance was 729 bp, which occurred between two ORFs located in the 2.2-kb inverted terminal repeat region of the PBCV-1 genome. Twenty-seven of the 85 major ORFs resemble proteins in databases, including the large subunit of ribonucleotide diphosphate reductase, ATP-dependent DNA ligase, type II DNA topoisomerase, a helicase, histidine decarboxylase, dCMP deaminase, dUTP pyrophosphatase, proliferating cell nuclear antigen, a transposase, fungal translation elongation factor 3 (EF-3), UDP glucose dehydrogenase, a protein kinase, and an adenine DNA methyltransferase and its corresponding DNA site-specific endonuclease. Seventeen of the 153 ORFs resembled other PBCV-1 ORFs, suggesting that they represent either gene duplications or gene families.
AB - This report completes a preliminary analysis of the sequence of the 330,740-bp chlorella virus PBCV-1 genome, the largest virus genome to be sequenced to date. The PBCV-1 genome is 57% the size of the genome from the smallest self-replicating organism, Mycoplasma genitalium. Analysis of 74 kb of newly sequenced DNA, from the right terminus of the PBCV-1 genome, revealed 153 open reading frames (ORFs) of 65 codons or longer. Eighty-five of these ORFs, which are evenly distributed on both strands of the DNA, were considered major ORFs. Fifty-nine of the major ORFs were separated by less than 100 bp. The largest intergenic distance was 729 bp, which occurred between two ORFs located in the 2.2-kb inverted terminal repeat region of the PBCV-1 genome. Twenty-seven of the 85 major ORFs resemble proteins in databases, including the large subunit of ribonucleotide diphosphate reductase, ATP-dependent DNA ligase, type II DNA topoisomerase, a helicase, histidine decarboxylase, dCMP deaminase, dUTP pyrophosphatase, proliferating cell nuclear antigen, a transposase, fungal translation elongation factor 3 (EF-3), UDP glucose dehydrogenase, a protein kinase, and an adenine DNA methyltransferase and its corresponding DNA site-specific endonuclease. Seventeen of the 153 ORFs resembled other PBCV-1 ORFs, suggesting that they represent either gene duplications or gene families.
UR - http://www.scopus.com/inward/record.url?scp=0030698072&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030698072&partnerID=8YFLogxK
U2 - 10.1006/viro.1997.8805
DO - 10.1006/viro.1997.8805
M3 - Article
C2 - 9356347
AN - SCOPUS:0030698072
SN - 0042-6822
VL - 237
SP - 360
EP - 377
JO - Virology
JF - Virology
IS - 2
ER -