TY - JOUR
T1 - Analysis of 76 kb of the chlorella virus PBCV-1 330-kb genome
T2 - Map positions 182 to 258
AU - Kutish, Gerald F.
AU - Li, Yu
AU - Lu, Zhiqiang
AU - Furuta, Masakazu
AU - Rock, Daniel L.
AU - Van Etten, James L.
N1 - Funding Information:
We thank Xiaofen Liao, Bonnie Skuba, and Dwight Burbank for technical help and Mike Nelson, Les Lane, Myron Brakke, Russ Meints, and Malcolm Brown for helpful discussions. This investigation was supported, in part, by Public Health Service Grant GM-32441 from the National Institute of General Medical Sciences to J.V.E. Y.L. was supported in part by a University of Nebraska–Lincoln Center of Biotechnology Graduate Research Fellowship.
PY - 1996/9/15
Y1 - 1996/9/15
N2 - Analysis of 76 kb of newly sequenced DNA, located between map positions 182 and 258 kb in the 330-kb chlorella virus PBCV-1 genome, revealed 175 open reading frames (ORFs) of 65 codons or longer. One hundred and five of these 175 ORFs were considered major ORFs. Twenty-one of the 105 major ORFs resembled proteins in databases including ribonucleotide reductase small subunit, RNase III, thioredoxin, glutaredoxin, protein disulfide isomerase, deoxynucleoside kinase, frog virus 3 ATPase, Acetobacter cellulose synthase, a bacteriophage encoded endonuclease, and two C-5 cytosine DNA methyltransferases. One of the ORFs was the PBCV-1 major capsid protein. The 105 major ORFs were evenly distributed along the genome. One set of ORFs was separated by 543 nucleotides whereas 75 of the ORFs were separated by fewer than 100 nucleotides. Nineteen of the 175 ORFs resembled other PBCV-1 ORFs, suggesting that they represent either gene duplications or gene families.
AB - Analysis of 76 kb of newly sequenced DNA, located between map positions 182 and 258 kb in the 330-kb chlorella virus PBCV-1 genome, revealed 175 open reading frames (ORFs) of 65 codons or longer. One hundred and five of these 175 ORFs were considered major ORFs. Twenty-one of the 105 major ORFs resembled proteins in databases including ribonucleotide reductase small subunit, RNase III, thioredoxin, glutaredoxin, protein disulfide isomerase, deoxynucleoside kinase, frog virus 3 ATPase, Acetobacter cellulose synthase, a bacteriophage encoded endonuclease, and two C-5 cytosine DNA methyltransferases. One of the ORFs was the PBCV-1 major capsid protein. The 105 major ORFs were evenly distributed along the genome. One set of ORFs was separated by 543 nucleotides whereas 75 of the ORFs were separated by fewer than 100 nucleotides. Nineteen of the 175 ORFs resembled other PBCV-1 ORFs, suggesting that they represent either gene duplications or gene families.
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U2 - 10.1006/viro.1996.0482
DO - 10.1006/viro.1996.0482
M3 - Article
C2 - 8806566
AN - SCOPUS:0030587386
SN - 0042-6822
VL - 223
SP - 303
EP - 317
JO - Virology
JF - Virology
IS - 2
ER -