TY - JOUR
T1 - Analysis of a bovine herpesvirus 1 protein encoded by an alternatively spliced latency related (LR) RNA that is abundantly expressed in latently infected neurons
AU - Sinani, Devis
AU - Liu, Yilin
AU - Jones, Clinton
N1 - Funding Information:
Drs. Devis Sinani and Yilin Liu contributed equally to these studies. This research was supported by grants from the Nebraska Research Initiative (072014) and the USDA , Agriculture and Food Research Initiative Competitive Grants Program ( 09-01653 and 2013-01041 ). A National Institutes of Health grant to the Nebraska Center for Virology ( 1P20RR15635 ), in particular, the Microscopy Core facility has also supported these studies. Devis Sinani was partially supported by a fellowship from a Ruth L. Kirschstein National Research Service Award 1 T32 AIO60547 ( National Institute of Allergy and Infectious Diseases ).
PY - 2014/9
Y1 - 2014/9
N2 - The bovine herpes virus 1 (BoHV-1) encoded latency-related RNA (LR-RNA) is abundantly expressed in latently infected sensory neurons. A LR mutant virus with three stop codons at the amino-terminus of ORF2 does not reactivate from latency or replicate efficiently in certain tissues. ORF2 inhibits apoptosis, interacts with Notch1 or Notch3, and interferes with Notch mediated signaling. Alternative splicing of LR-RNA in trigeminal ganglia yields transcripts that have the potential to encode a protein containing most of ORF2 sequences and parts of other coding sequences located within the LR gene. In this study, we determined that an ORF2 protein fused with reading frame B (15d ORF) was more stable in transfected cells. ORF2 and the 15d ORF stimulated neurite formation in mouse neuroblastoma cells, interfered with Notch3 mediated trans-activation, and had similar DNA binding properties. Increased stability of the 15d ORF is predicted to enhance the establishment of latency.
AB - The bovine herpes virus 1 (BoHV-1) encoded latency-related RNA (LR-RNA) is abundantly expressed in latently infected sensory neurons. A LR mutant virus with three stop codons at the amino-terminus of ORF2 does not reactivate from latency or replicate efficiently in certain tissues. ORF2 inhibits apoptosis, interacts with Notch1 or Notch3, and interferes with Notch mediated signaling. Alternative splicing of LR-RNA in trigeminal ganglia yields transcripts that have the potential to encode a protein containing most of ORF2 sequences and parts of other coding sequences located within the LR gene. In this study, we determined that an ORF2 protein fused with reading frame B (15d ORF) was more stable in transfected cells. ORF2 and the 15d ORF stimulated neurite formation in mouse neuroblastoma cells, interfered with Notch3 mediated trans-activation, and had similar DNA binding properties. Increased stability of the 15d ORF is predicted to enhance the establishment of latency.
KW - Alternative splicing
KW - Bovine herpesvirus 1
KW - Latency in sensory neurons
KW - Notch mediated signaling
KW - ORF2
UR - http://www.scopus.com/inward/record.url?scp=84905372685&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84905372685&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2014.06.038
DO - 10.1016/j.virol.2014.06.038
M3 - Article
C2 - 25104616
AN - SCOPUS:84905372685
VL - 464-465
SP - 244
EP - 252
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -