Analysis of biological interactions by affinity chromatography: Clinical and pharmaceutical applications

David S. Hage

Research output: Contribution to journalReview articlepeer-review

29 Scopus citations


Background: The interactions between biochemical and chemical agents in the body are important in many clinical processes. Affinity chromatography and highperformance affinity chromatography (HPAC), in which a column contains an immobilized biologically related binding agent, are 2 methods that can be used to study these interactions. Content: This review presents various approaches that can be used in affinity chromatography and HPAC to characterize the strength or rate of a biological interaction, the number and types of sites that are involved in this process, and the interactions between multiple solutes for the same binding agent. A number of applications for these methods are examined, with an emphasis on recent developments and high-performance affinity methods. These applications include the use of these techniques for fundamental studies of biological interactions, high-throughput screening of drugs, work with modified proteins, tools for personalized medicine, and studies of drug-drug competition for a common binding agent. Summary: The wide range of formats and detection methods that can be used with affinity chromatography and HPAC for examining biological interactions makes these tools attractive for various clinical and pharmaceutical applications. Future directions in the development of small-scale columns and the coupling of these methods with other techniques, such as mass spectrometry or other separation methods, should continue to increase the flexibility and ease with which these approaches can be used in work involving clinical or pharmaceutical samples.

Original languageEnglish (US)
Pages (from-to)1083-1093
Number of pages11
JournalClinical Chemistry
Issue number6
StatePublished - Jun 2017

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical


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