Analysis of chromatin interactions mediated by specific architectural proteins in drosophila cells

Masami Ando-Kuri, I. Sarahi M. Rivera, M. Jordan Rowley, Victor G. Corces

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Chromosome conformation capture assays have been established, modified, and enhanced for over a decade with the purpose of studying nuclear organization. A recently published method uses in situ Hi-C followed by chromatin immunoprecipitation (HiChIP) to enrich the overall yield of significant genome-wide interactions mediated by a specific protein. Here we applied a modified version of the HiChIP protocol to retrieve the significant contacts mediated by architectural protein CP190 in D. melanogaster cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages239-256
Number of pages18
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1766
ISSN (Print)1064-3745

Keywords

  • Chromatin architecture
  • Chromatin immunoprecipitation
  • Epigenetics
  • HiChIP
  • In situ Hi-C
  • Transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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