Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus

Toyoko Tsukuda, Kelly M. Trujillo, Emmanuelle Martini, Mary Ann Osley

Research output: Contribution to journalReview article

5 Scopus citations

Abstract

DNA repair occurs in a chromatin context, and nucleosome remodeling is now recognized as an important regulatory feature by allowing repair factors access to damaged sites. The yeast mating type locus (MAT) has emerged an excellent model to study the role of chromatin remodeling at a well-defined DNA double-strand break (DSB). We discuss methods to study nucleosome dynamics and DSB repair factor recruitment to the MAT locus after a DSB has been formed.

Original languageEnglish (US)
Pages (from-to)40-45
Number of pages6
JournalMethods
Volume48
Issue number1
DOIs
StatePublished - May 1 2009

Keywords

  • Chromatin remodeling
  • DNA double-strand breaks
  • Yeast

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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