Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus

Toyoko Tsukuda; Kelly M. Trujillo; Emmanuelle Martini; Mary Ann Osley

doi:10.1016/j.ymeth.2009.02.007

Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus

Toyoko Tsukuda, Kelly M. Trujillo, Emmanuelle Martini, Mary Ann Osley

Research output: Contribution to journal › Review article › peer-review

7 Scopus citations

Abstract

DNA repair occurs in a chromatin context, and nucleosome remodeling is now recognized as an important regulatory feature by allowing repair factors access to damaged sites. The yeast mating type locus (MAT) has emerged an excellent model to study the role of chromatin remodeling at a well-defined DNA double-strand break (DSB). We discuss methods to study nucleosome dynamics and DSB repair factor recruitment to the MAT locus after a DSB has been formed.

Original language	English (US)
Pages (from-to)	40-45
Number of pages	6
Journal	Methods
Volume	48
Issue number	1
DOIs	https://doi.org/10.1016/j.ymeth.2009.02.007
State	Published - May 2009
Externally published	Yes

Keywords

Chromatin remodeling
DNA double-strand breaks
Yeast

ASJC Scopus subject areas

Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)

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10.1016/j.ymeth.2009.02.007

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title = "Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus",

abstract = "DNA repair occurs in a chromatin context, and nucleosome remodeling is now recognized as an important regulatory feature by allowing repair factors access to damaged sites. The yeast mating type locus (MAT) has emerged an excellent model to study the role of chromatin remodeling at a well-defined DNA double-strand break (DSB). We discuss methods to study nucleosome dynamics and DSB repair factor recruitment to the MAT locus after a DSB has been formed.",

keywords = "Chromatin remodeling, DNA double-strand breaks, Yeast",

author = "Toyoko Tsukuda and Trujillo, {Kelly M.} and Emmanuelle Martini and Osley, {Mary Ann}",

note = "Funding Information: Research in the authors{\textquoteright} laboratories is supported by grants from the NIH (CA118537; M.A.O.) and the CEA and CNRS (E.M.).",

year = "2009",

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doi = "10.1016/j.ymeth.2009.02.007",

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pages = "40--45",

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T1 - Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus

AU - Tsukuda, Toyoko

AU - Trujillo, Kelly M.

AU - Martini, Emmanuelle

AU - Osley, Mary Ann

N1 - Funding Information: Research in the authors’ laboratories is supported by grants from the NIH (CA118537; M.A.O.) and the CEA and CNRS (E.M.).

PY - 2009/5

Y1 - 2009/5

N2 - DNA repair occurs in a chromatin context, and nucleosome remodeling is now recognized as an important regulatory feature by allowing repair factors access to damaged sites. The yeast mating type locus (MAT) has emerged an excellent model to study the role of chromatin remodeling at a well-defined DNA double-strand break (DSB). We discuss methods to study nucleosome dynamics and DSB repair factor recruitment to the MAT locus after a DSB has been formed.

AB - DNA repair occurs in a chromatin context, and nucleosome remodeling is now recognized as an important regulatory feature by allowing repair factors access to damaged sites. The yeast mating type locus (MAT) has emerged an excellent model to study the role of chromatin remodeling at a well-defined DNA double-strand break (DSB). We discuss methods to study nucleosome dynamics and DSB repair factor recruitment to the MAT locus after a DSB has been formed.

KW - Chromatin remodeling

KW - DNA double-strand breaks

KW - Yeast

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U2 - 10.1016/j.ymeth.2009.02.007

DO - 10.1016/j.ymeth.2009.02.007

M3 - Review article

C2 - 19245836

AN - SCOPUS:65649142756

SN - 1046-2023

VL - 48

SP - 40

EP - 45

JO - Methods

JF - Methods

IS - 1

ER -

Analysis of chromatin remodeling during formation of a DNA double-strand break at the yeast mating type locus

Abstract

Keywords

ASJC Scopus subject areas

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