TY - JOUR
T1 - Analysis of cyclins in trigeminal ganglia of calves infected with bovine herpesvirus-1
AU - Winkler, M. T.
AU - Schang, L. S.
AU - Doster, A.
AU - Holt, T.
AU - Jones, C.
PY - 2000
Y1 - 2000
N2 - Following acute infection of cattle with bovine herpesvirus-1 (BHV-1), cyclin expression was examined in trigeminal ganglia (TG). Cyclin A was primarily detected in the nucleus of TG neurons. In contrast, cyclin D1 and cyclin E were detected in the nucleus and cytoplasm of TG neurons. Uninfected or latently infected calves did not express detectable levels of these cyclins in TG neurons. Following dexamethasone-induced reactivation, cyclins D1, E and A were also detected in TG neurons. In situ hybridization of consecutive sections demonstrated that many neurons expressing cyclins contained viral nucleic acid, demonstrating that they were infected. Based on these observations, we hypothesize that BHV-1 infection activates neuronal cyclin expression to enhance productive infection. It is also possible that the stress of neuronal infection or reactivation leads to cyclin expression.
AB - Following acute infection of cattle with bovine herpesvirus-1 (BHV-1), cyclin expression was examined in trigeminal ganglia (TG). Cyclin A was primarily detected in the nucleus of TG neurons. In contrast, cyclin D1 and cyclin E were detected in the nucleus and cytoplasm of TG neurons. Uninfected or latently infected calves did not express detectable levels of these cyclins in TG neurons. Following dexamethasone-induced reactivation, cyclins D1, E and A were also detected in TG neurons. In situ hybridization of consecutive sections demonstrated that many neurons expressing cyclins contained viral nucleic acid, demonstrating that they were infected. Based on these observations, we hypothesize that BHV-1 infection activates neuronal cyclin expression to enhance productive infection. It is also possible that the stress of neuronal infection or reactivation leads to cyclin expression.
UR - http://www.scopus.com/inward/record.url?scp=0034535138&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034535138&partnerID=8YFLogxK
U2 - 10.1099/0022-1317-81-12-2993
DO - 10.1099/0022-1317-81-12-2993
M3 - Article
C2 - 11086130
AN - SCOPUS:0034535138
SN - 0022-1317
VL - 81
SP - 2993
EP - 2998
JO - Journal of General Virology
JF - Journal of General Virology
IS - 12
ER -