Analysis of drug–protein interactions by high-performance affinity chromatography: Interactions of sulfonylurea drugs with normal and glycated human serum albumin

Ryan Matsuda, Jeanethe Anguizola, Krina S. Hoy, David S. Hage

Research output: Contribution to journalArticle

11 Scopus citations


High-performance affinity chromatography (HPAC) is a type of liquid chromatography that has seen growing use as a tool for the study of drug–protein interactions. This report describes how HPAC can be used to provide information on the number of binding sites, equilibrium constants, and changes in binding that can occur during drug–protein interactions. This approach will be illustrated through recent data that have been obtained by HPAC for the binding of sulfonylurea drugs and other solutes to the protein human serum albumin (HSA), and especially to forms of this protein that have been modified by non-enzymatic glycation. The theory and use of both frontal analysis and zonal elution competition studies in such work will be discussed. Various practical aspects of these experiments will be presented, as well as factors to consider in the extension of these methods to other drugs and proteins or additional types of biological interactions.

Original languageEnglish (US)
Pages (from-to)255-277
Number of pages23
JournalMethods in Molecular Biology
StatePublished - Jan 1 2015



  • Biointeraction analysis
  • Drug–protein binding
  • Frontal analysis
  • Glycation
  • High-performance affinity chromatography
  • Human serum albumin
  • Sulfonylurea drugs
  • Zonal elution

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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