Analysis of keratinocytes stiffness after desmosome disruption using Atomic Force Microscopy based nanomanipulation

Ruiguo Yang, Ning Xi, Carmen Kar, Man Fung, King Wai, Chiu Lai, Kristina Seiffert-Sinha, Amnish A. Sinha

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Scopus citations

Abstract

Desmosomes are cell-cell junctions that provide mechanical strength for cells and maintain integrity of tissues in animal skins and hearts. Disruption of the desmosome like in some autoimmune diseases will therefore change the mechanical property of the anchoring cells and causing blistering. In the paper, we used Atomic Force Microscopy (AFM) to visualize the keratinocytes intercellular structure. The AFM based nanomanipulation system is employed to perform the nanoindentation experiment for measuring keratinocytes nanomechanical property under both physiological and pathophysiological conditions. Our demonstration of the use of AFM for in situ imaging and nanomanipulation for quantifying stiffness in nanoscale can facilitate the investigation of diseases related to blistering and development of therapeutic strategies accordingly.

Original languageEnglish (US)
Title of host publication2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009
Pages640-643
Number of pages4
StatePublished - Dec 1 2009
Event2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009 - Genoa, Italy
Duration: Jul 26 2009Jul 30 2009

Publication series

Name2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009

Other

Other2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009
CountryItaly
CityGenoa
Period7/26/097/30/09

Keywords

  • AFM
  • Cell stiffness
  • Desmosome
  • Nanomanipulation

ASJC Scopus subject areas

  • Process Chemistry and Technology
  • Electrical and Electronic Engineering

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    Yang, R., Xi, N., Kar, C., Fung, M., Wai, K., Lai, C., Seiffert-Sinha, K., & Sinha, A. A. (2009). Analysis of keratinocytes stiffness after desmosome disruption using Atomic Force Microscopy based nanomanipulation. In 2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009 (pp. 640-643). [5394778] (2009 9th IEEE Conference on Nanotechnology, IEEE NANO 2009).