Analysis of nonsense-mediated mrna decay in saccharomyces cerevisiae

Bessie W. Kebaara; Kristian E. Baker; Krista D. Patefield; Audrey L. Atkin

doi:10.1002/0471143030.cb2703s54

Analysis of nonsense-mediated mrna decay in saccharomyces cerevisiae

Bessie W. Kebaara, Kristian E. Baker, Krista D. Patefield, Audrey L. Atkin

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Nonsense-mediated mRNA decay is a highly conserved pathway that degrades mRNAs with premature termination codons. These mRNAs include mRNAs transcribed from nonsense or frameshift alleles as well as wild-type mRNA with signals that direct ribosomes to terminate prematurely. This unit describes techniques to monitor steady-state mRNA levels, decay rates, and structural features of mRNAs targeted by this pathway, as well as in vivo analysis of nonsense suppression and allosuppression in the yeast Saccharomyces cerevisiae. Protocols for the structural features of mRNA include analysis of cap status, 5' and 3' untranslated region (UTR) lengths, and poly(A) tail length.

Original language	English (US)
Article number	27.3
Journal	Current Protocols in Cell Biology
Issue number	SUPPL.54
DOIs	https://doi.org/10.1002/0471143030.cb2703s54
State	Published - Mar 2012
Externally published	Yes

Keywords

5'-cap
Allosuppression
Nonsense suppression
Poly(A) tail length
Steady-state mRNA level
Untranslated region length
mRNA decay rate

ASJC Scopus subject areas

Cell Biology

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10.1002/0471143030.cb2703s54

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title = "Analysis of nonsense-mediated mrna decay in saccharomyces cerevisiae",

abstract = "Nonsense-mediated mRNA decay is a highly conserved pathway that degrades mRNAs with premature termination codons. These mRNAs include mRNAs transcribed from nonsense or frameshift alleles as well as wild-type mRNA with signals that direct ribosomes to terminate prematurely. This unit describes techniques to monitor steady-state mRNA levels, decay rates, and structural features of mRNAs targeted by this pathway, as well as in vivo analysis of nonsense suppression and allosuppression in the yeast Saccharomyces cerevisiae. Protocols for the structural features of mRNA include analysis of cap status, 5' and 3' untranslated region (UTR) lengths, and poly(A) tail length.",

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AU - Baker, Kristian E.

AU - Patefield, Krista D.

AU - Atkin, Audrey L.

PY - 2012/3

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AB - Nonsense-mediated mRNA decay is a highly conserved pathway that degrades mRNAs with premature termination codons. These mRNAs include mRNAs transcribed from nonsense or frameshift alleles as well as wild-type mRNA with signals that direct ribosomes to terminate prematurely. This unit describes techniques to monitor steady-state mRNA levels, decay rates, and structural features of mRNAs targeted by this pathway, as well as in vivo analysis of nonsense suppression and allosuppression in the yeast Saccharomyces cerevisiae. Protocols for the structural features of mRNA include analysis of cap status, 5' and 3' untranslated region (UTR) lengths, and poly(A) tail length.

KW - 5'-cap

KW - Allosuppression

KW - Nonsense suppression

KW - Poly(A) tail length

KW - Steady-state mRNA level

KW - Untranslated region length

KW - mRNA decay rate

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Analysis of nonsense-mediated mrna decay in saccharomyces cerevisiae

Abstract

Keywords

ASJC Scopus subject areas

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