Analysis of oxytetracycline, tetracycline, and chlortetracycline in water using solid-phase extraction and liquid chromatography-tandem mass spectrometry

J. Zhu; D. D. Snow; D. A. Cassada; S. J. Monson; R. F. Spalding

doi:10.1016/S0021-9673(01)01139-6

Analysis of oxytetracycline, tetracycline, and chlortetracycline in water using solid-phase extraction and liquid chromatography-tandem mass spectrometry

J. Zhu, D. D. Snow, D. A. Cassada, S. J. Monson, R. F. Spalding

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

A method using liquid chromatography-tandem mass spectrometry has been developed for determination of trace levels of tetracycline antibiotics in ground water and confined animal feeding operation waste water. Oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) were extracted from water samples using both polymeric and C₁₈ extraction cartridges. The addition of a buffer containing potassium phosphate and citric acid improved tetracycline recoveries in lagoon water. Method detection limits determined in reagent water fortified with 1 μg l^-1 OTC, TC, and CTC were 0.21, 0.20, and 0.28 μg l^-1. Method detection limits in lagoon water samples fortified at 20 μg l^-1 for OTC, TC, and CTC were 3.6, 3.1, and 3.8 μg l^-1. Variability in recovery from laboratory fortified blanks ranged from 86 to 110% during routine analysis.

Original language	English (US)
Pages (from-to)	177-186
Number of pages	10
Journal	Journal of Chromatography A
Volume	928
Issue number	2
DOIs	https://doi.org/10.1016/S0021-9673(01)01139-6
State	Published - Sep 14 2001

Keywords

Chlortetracycline
Oxytetracycline
Tetracycline
Water analysis

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Organic Chemistry

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10.1016/S0021-9673(01)01139-6

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title = "Analysis of oxytetracycline, tetracycline, and chlortetracycline in water using solid-phase extraction and liquid chromatography-tandem mass spectrometry",

abstract = "A method using liquid chromatography-tandem mass spectrometry has been developed for determination of trace levels of tetracycline antibiotics in ground water and confined animal feeding operation waste water. Oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) were extracted from water samples using both polymeric and C18 extraction cartridges. The addition of a buffer containing potassium phosphate and citric acid improved tetracycline recoveries in lagoon water. Method detection limits determined in reagent water fortified with 1 μg l-1 OTC, TC, and CTC were 0.21, 0.20, and 0.28 μg l-1. Method detection limits in lagoon water samples fortified at 20 μg l-1 for OTC, TC, and CTC were 3.6, 3.1, and 3.8 μg l-1. Variability in recovery from laboratory fortified blanks ranged from 86 to 110% during routine analysis.",

keywords = "Chlortetracycline, Oxytetracycline, Tetracycline, Water analysis",

author = "J. Zhu and Snow, {D. D.} and Cassada, {D. A.} and Monson, {S. J.} and Spalding, {R. F.}",

note = "Funding Information: Support for this research was provided by the Nebraska Department of Environmental Quality (NDEQ). The LC–MS–MS instrumentation was in part purchased with Nebraska Research Initiative (NRI) funds for the core laboratory. We thank Dr Glen Martin and Mari Sadayappan for providing the lagoon water samples, and Jeff Toavs for his assistance in groundwater sample collection. Thanks also to Steve Comfort and Dennis McCallister for their review of this manuscript. This article has been assigned Journal Series No. 13304, Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska. ",

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AU - Snow, D. D.

AU - Cassada, D. A.

AU - Monson, S. J.

AU - Spalding, R. F.

N1 - Funding Information: Support for this research was provided by the Nebraska Department of Environmental Quality (NDEQ). The LC–MS–MS instrumentation was in part purchased with Nebraska Research Initiative (NRI) funds for the core laboratory. We thank Dr Glen Martin and Mari Sadayappan for providing the lagoon water samples, and Jeff Toavs for his assistance in groundwater sample collection. Thanks also to Steve Comfort and Dennis McCallister for their review of this manuscript. This article has been assigned Journal Series No. 13304, Agricultural Research Division, Institute of Agriculture and Natural Resources, University of Nebraska.

PY - 2001/9/14

Y1 - 2001/9/14

N2 - A method using liquid chromatography-tandem mass spectrometry has been developed for determination of trace levels of tetracycline antibiotics in ground water and confined animal feeding operation waste water. Oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) were extracted from water samples using both polymeric and C18 extraction cartridges. The addition of a buffer containing potassium phosphate and citric acid improved tetracycline recoveries in lagoon water. Method detection limits determined in reagent water fortified with 1 μg l-1 OTC, TC, and CTC were 0.21, 0.20, and 0.28 μg l-1. Method detection limits in lagoon water samples fortified at 20 μg l-1 for OTC, TC, and CTC were 3.6, 3.1, and 3.8 μg l-1. Variability in recovery from laboratory fortified blanks ranged from 86 to 110% during routine analysis.

AB - A method using liquid chromatography-tandem mass spectrometry has been developed for determination of trace levels of tetracycline antibiotics in ground water and confined animal feeding operation waste water. Oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) were extracted from water samples using both polymeric and C18 extraction cartridges. The addition of a buffer containing potassium phosphate and citric acid improved tetracycline recoveries in lagoon water. Method detection limits determined in reagent water fortified with 1 μg l-1 OTC, TC, and CTC were 0.21, 0.20, and 0.28 μg l-1. Method detection limits in lagoon water samples fortified at 20 μg l-1 for OTC, TC, and CTC were 3.6, 3.1, and 3.8 μg l-1. Variability in recovery from laboratory fortified blanks ranged from 86 to 110% during routine analysis.

KW - Chlortetracycline

KW - Oxytetracycline

KW - Tetracycline

KW - Water analysis

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Analysis of oxytetracycline, tetracycline, and chlortetracycline in water using solid-phase extraction and liquid chromatography-tandem mass spectrometry

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Keywords

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