Analysis of reactive carbonyls in the expired air of transgenic mice

Susan E. Ebeler, Steven H. Hinrichs, Andrew J. Clifford, Takayuki Shibamoto

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Methods for the determination of trace levels of volatile carbonyl compounds in air expired from mice were developed and validated. Tumor bearing transgenic mice or nontransgenic control mice were placed into a glass chamber through which air was passed continuously at 90 ml/min for 1 h. The effluent gas stream was bubbled into an aqueous cysteamine solution or an aqueous methylhydrazinesolution. Formaldehyde, acetaldehyde, and acetone in expired air were derivatized to thiazolidines with cysteamine and malonaldehyde was derivatized to 1-methyl-2-pyrazole with methylhydrazine. The derivatized compounds were analyzed by capillary gas chromatography with flame photometric or nitrogen-phosphorous-specific detection. The lowest level quantitated was 4 μg/ml thiazolidine, equivalent to 1.35 μg/ml formaldehyde. Formaldehyde was recovered at a level of 1356 ± 234 nmol/kg0.75 (mean ± SD) from mice with tumors and 898 ± 97 nmol/kg0.75 from mice without tumors, suggesting that tumor bearing transgenic mice expired significantly more formaldehyde than did tumor free controls. Amounts of expired acetaldehyde and acetone were not different among mice. Malonaldehyde was not detected in either group of mice.

Original languageEnglish (US)
Pages (from-to)183-186
Number of pages4
JournalAnalytical Biochemistry
Volume205
Issue number1
DOIs
StatePublished - Aug 15 1992

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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