Analysis of sulfates on low molecular weight heparin using mass spectrometry: structural characterization of enoxaparin

Rohitesh Gupta, Moorthy P. Ponnusamy

Research output: Contribution to journalReview article

1 Scopus citations

Abstract

Introduction: Structural characterization of low molecular weight heparin (LMWH) is critical to meet biosimilarity standards. In this context, the review focuses on structural analysis of labile sulfates attached to the side-groups of LMWH using mass spectrometry. A comprehensive review of this topic will help readers to identify key strategies for tackling the problem related to sulfate loss. At the same time, various mass spectrometry techniques are presented to facilitate compositional analysis of LMWH, mainly enoxaparin. Areas covered: This review summarizes findings on mass spectrometry application for LMWH, including modulation of sulfates, using enzymology and sample preparation approaches. Furthermore, popular open-source software packages for automated spectral data interpretation are also discussed. Successful use of LC/MS can decipher structural composition for LMWH and help evaluate their sameness or biosimilarity with the innovator molecule. Overall, the literature has been searched using PubMed by typing various search queries such as ‘enoxaparin’, ‘mass spectrometry’, ‘low molecular weight heparin’, ‘structural characterization’, etc. Expert commentary: This section highlights clinically relevant areas that need improvement to achieve satisfactory commercialization of LMWHs. It also primarily emphasizes the advancements in instrumentation related to mass spectrometry, and discusses building automated software for data interpretation and analysis.

Original languageEnglish (US)
Pages (from-to)503-513
Number of pages11
JournalExpert Review of Proteomics
Volume15
Issue number6
DOIs
StatePublished - Jun 3 2018

Keywords

  • Low molecular weight heparin
  • enoxaparin
  • mass spectrometry
  • size exclusion chromatography
  • sulfolyltransferase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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