TY - JOUR
T1 - Analysis of the initial stages of plant protoplast development using 33258 Hoechst
T2 - reactivation of the cell cycle
AU - Galbraith, David W.
AU - Mauch, Teri J.
AU - Shields, Barbara A.
PY - 1981/4
Y1 - 1981/4
N2 - A microfluorimetric procedure, employing the fluorescent stain 33258 Hoechst, has been developed for the investigation of the process of DNA synthesis during the initial stages of culture of tobacco (N. tabacum cv. Xanthi) leaf protoplasts. In this system, the freshly‐isolated protoplasts exhibited a unimodal distribution of nuclear DNA content characteristic of the diploid state. The almost immediate onset of DNA synthesis during culture resulted in a doubling of nuclear DNA levels prior to the first mitoses. Although the majority of the protoplasts subsequently entered into synchronous mitosis and cell division, a proportion of the remainder developed into large polyploid cells. Upon further culture, the polyploid cells became subdivided into clusters of small diploid cells. Measurement of total cell protein and cell volumes during culture indicated that a relationship existed between these parameters and the initiation of mitosis. The significance of these observations is discussed.
AB - A microfluorimetric procedure, employing the fluorescent stain 33258 Hoechst, has been developed for the investigation of the process of DNA synthesis during the initial stages of culture of tobacco (N. tabacum cv. Xanthi) leaf protoplasts. In this system, the freshly‐isolated protoplasts exhibited a unimodal distribution of nuclear DNA content characteristic of the diploid state. The almost immediate onset of DNA synthesis during culture resulted in a doubling of nuclear DNA levels prior to the first mitoses. Although the majority of the protoplasts subsequently entered into synchronous mitosis and cell division, a proportion of the remainder developed into large polyploid cells. Upon further culture, the polyploid cells became subdivided into clusters of small diploid cells. Measurement of total cell protein and cell volumes during culture indicated that a relationship existed between these parameters and the initiation of mitosis. The significance of these observations is discussed.
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U2 - 10.1111/j.1399-3054.1981.tb05573.x
DO - 10.1111/j.1399-3054.1981.tb05573.x
M3 - Article
AN - SCOPUS:0000472740
SN - 0031-9317
VL - 51
SP - 380
EP - 386
JO - Physiologia Plantarum
JF - Physiologia Plantarum
IS - 4
ER -