TY - JOUR
T1 - Analysis of the mom1 modifier of intestinal neoplasia in mice
AU - Gould, Karen A.
AU - Dove, William F.
N1 - Funding Information:
We gratefully acknowledge the contributions of past and present members of both the Dove laboratory and the laboratory of Eric Lander, who have participated in the investigations of Min and Moml. This is publication number 3507 from the Laboratory of Genetics. This work is supported by the National Cancer Institute through Grants CA50585, CA63677, Core Grant CA07075, and Training Grant CA09135.
PY - 1998
Y1 - 1998
N2 - Although the methodology for mapping genes controlling susceptibility to tumor development in mice is becoming well established, it remains a formidable challenge to move from linkage to locus. Positional cloning, now commonly used in the identification of loci affecting a qualitative phenotype, has yet to be successfully applied to quantitative trait loci. This study describes the application of candidate gene testing, a method complementary to positional cloning. The method has been applied to evaluate candidates for the quantitative trait locus, Mom1, which modifies the susceptibility of Apc(Min/+) mice to spontaneous intestinal tumor development. The authors also discuss the further testing of one candidate, the phospholipase gene Pla2g2a, by transgenesis. Finally, studies on the mode of action of Moral are discussed in light of the evidence that Moral encodes this secretory phospholipase.
AB - Although the methodology for mapping genes controlling susceptibility to tumor development in mice is becoming well established, it remains a formidable challenge to move from linkage to locus. Positional cloning, now commonly used in the identification of loci affecting a qualitative phenotype, has yet to be successfully applied to quantitative trait loci. This study describes the application of candidate gene testing, a method complementary to positional cloning. The method has been applied to evaluate candidates for the quantitative trait locus, Mom1, which modifies the susceptibility of Apc(Min/+) mice to spontaneous intestinal tumor development. The authors also discuss the further testing of one candidate, the phospholipase gene Pla2g2a, by transgenesis. Finally, studies on the mode of action of Moral are discussed in light of the evidence that Moral encodes this secretory phospholipase.
KW - Adenomatous polyposis coli (Apc)
KW - Intestinal tumors
KW - Mice
KW - Modifier of Min- 1 (Mom1)
KW - Multiple intestinal neoplasia (Min)
KW - Quantitative trait locus
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U2 - 10.3109/01902149809087379
DO - 10.3109/01902149809087379
M3 - Article
C2 - 9659576
AN - SCOPUS:0031832644
SN - 0190-2148
VL - 24
SP - 437
EP - 453
JO - Experimental Lung Research
JF - Experimental Lung Research
IS - 4
ER -