Analysis of the N-glycans of recombinant human Factor IX purified from transgenic pig milk

Geun Cheol Gil, William H. Velander, Kevin E. Van Cott

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Glycosylation of recombinant proteins is of particular importance because it can play significant roles in the clinical properties of the glycoprotein. In this work, the N-glycan structures of recombinant human Factor IX (tg-FIX) produced in the transgenic pig mammary gland were determined. The majority of the N-glycans of transgenic pig-derived Factor IX (tg-FIX) are complex, bi-antennary with one or two terminal N -acetylneuraminic acid (Neu5Ac) moieties. We also found that the N-glycan structures of tg-FIX produced in the porcine mammary epithelial cells differed with respect to N-glycans from glycoproteins produced in other porcine tissues. tg-FIX contains no detectable Neu5Gc, the sialic acid commonly found in porcine glycoproteins produced in other tissues. Additionally, we were unable to detect glycans in tg-FIX that have a terminal Galα(1,3)Gal disaccharide sequence, which is strongly antigenic in humans. The N-glycan structures of tg-FIX are also compared to the published N-glycan structures of recombinant human glycoproteins produced in other transgenic animal species. While tg-FIX contains only complex structures, antithrombin III (goat), C1 inhibitor (rabbit), and lactoferrin (cow) have both high mannose and complex structures. Collectively, these data represent a beginning point for the future investigation of species-specific and tissue/cell-specific differences in N-glycan structures among animals used for transgenic animal bioreactors. The Author 2008. Published by Oxford University Press. All rights reserved.

Original languageEnglish (US)
Pages (from-to)526-539
Number of pages14
Issue number7
StatePublished - Jul 2008
Externally publishedYes


  • Factor IX
  • Glycoprotein
  • Glycosylation
  • Mammary gland
  • Transgenic animal

ASJC Scopus subject areas

  • Biochemistry


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