Abstract
Uridylation (3′ untemplated uridine addition) provides a mechanism to trigger the degradation of miRNAs and the 5′ cleavage products (5′ CP) that are produced from miRNA-directed ARGONAUTE (AGO) cleavage of target RNAs. We have recently shown that HEN1 SUPPRESSOR 1 (HESO1), a terminal uridylyltransferase, and its homolog UTP:RNA uridylyltransferase 1 (URT1) catalyze the uridylation of miRNAs and 5′ CPs within the AGO complex in higher plants. In this chapter, we describe detailed protocols for analyzing 3′ end uridylation of both AGO-bound miRNAs and 5′ CP.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 23-37 |
| Number of pages | 15 |
| DOIs | |
| State | Published - 2017 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1640 |
| ISSN (Print) | 1064-3745 |
Keywords
- 5′ Cleavage product
- ARGONAUTE
- HESO1
- Rapid amplification of cDNA ends (RACE)
- Uridylation
- miRNA
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Ren, G., Wang, X., & Yu, B. (2017). Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants. In Methods in Molecular Biology (pp. 23-37). (Methods in Molecular Biology; Vol. 1640). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7165-7_2