@inbook{9c44bff8e21247879d078f8a18c7a4bb,
title = "Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants",
abstract = "Uridylation (3′ untemplated uridine addition) provides a mechanism to trigger the degradation of miRNAs and the 5′ cleavage products (5′ CP) that are produced from miRNA-directed ARGONAUTE (AGO) cleavage of target RNAs. We have recently shown that HEN1 SUPPRESSOR 1 (HESO1), a terminal uridylyltransferase, and its homolog UTP:RNA uridylyltransferase 1 (URT1) catalyze the uridylation of miRNAs and 5′ CPs within the AGO complex in higher plants. In this chapter, we describe detailed protocols for analyzing 3′ end uridylation of both AGO-bound miRNAs and 5′ CP.",
keywords = "5′ Cleavage product, ARGONAUTE, HESO1, Rapid amplification of cDNA ends (RACE), Uridylation, miRNA",
author = "Guodong Ren and Xiaoyan Wang and Bin Yu",
note = "Funding Information: We thank Yuan Wang for helping with the preparation of the figure. This work was supported by the Ministry of Science and Technology of China (Grant No. 2016YFA0503200) and National Natural Science Foundation of China (Grant Nos. 31471221 and 31622009) to G.R. and Nebraska Soybean Board (Grant No. 16R?05?3/3 #1706) and National Science Foundation (Award No. OIA?1557417) to B.Y. Publisher Copyright: {\textcopyright} 2017, Springer Science+Business Media LLC.",
year = "2017",
doi = "10.1007/978-1-4939-7165-7_2",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "23--37",
booktitle = "Methods in Molecular Biology",
}