Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants

Guodong Ren, Xiaoyan Wang, Bin Yu

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Uridylation (3′ untemplated uridine addition) provides a mechanism to trigger the degradation of miRNAs and the 5′ cleavage products (5′ CP) that are produced from miRNA-directed ARGONAUTE (AGO) cleavage of target RNAs. We have recently shown that HEN1 SUPPRESSOR 1 (HESO1), a terminal uridylyltransferase, and its homolog UTP:RNA uridylyltransferase 1 (URT1) catalyze the uridylation of miRNAs and 5′ CPs within the AGO complex in higher plants. In this chapter, we describe detailed protocols for analyzing 3′ end uridylation of both AGO-bound miRNAs and 5′ CP.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages23-37
Number of pages15
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1640
ISSN (Print)1064-3745

Keywords

  • 5′ Cleavage product
  • ARGONAUTE
  • HESO1
  • Rapid amplification of cDNA ends (RACE)
  • Uridylation
  • miRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants'. Together they form a unique fingerprint.

Cite this