@inbook{9c44bff8e21247879d078f8a18c7a4bb,
title = "Analysis of the uridylation of both ARGONAUTE-bound miRNAs and 5′ cleavage products of their target RNAs in plants",
abstract = "Uridylation (3′ untemplated uridine addition) provides a mechanism to trigger the degradation of miRNAs and the 5′ cleavage products (5′ CP) that are produced from miRNA-directed ARGONAUTE (AGO) cleavage of target RNAs. We have recently shown that HEN1 SUPPRESSOR 1 (HESO1), a terminal uridylyltransferase, and its homolog UTP:RNA uridylyltransferase 1 (URT1) catalyze the uridylation of miRNAs and 5′ CPs within the AGO complex in higher plants. In this chapter, we describe detailed protocols for analyzing 3′ end uridylation of both AGO-bound miRNAs and 5′ CP.",
keywords = "5′ Cleavage product, ARGONAUTE, HESO1, Rapid amplification of cDNA ends (RACE), Uridylation, miRNA",
author = "Guodong Ren and Xiaoyan Wang and Bin Yu",
year = "2017",
doi = "10.1007/978-1-4939-7165-7_2",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "23--37",
booktitle = "Methods in Molecular Biology",
}