Analysis of tumor-associated mucin glycotopes by western transfer methods

Vinayaga S. Gnanapragassam, Maneesh Jain, Surinder K. Batra

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

In mucins, glycosylation is complex and the most predominant posttranslational modification. Since mucins exhibit differential glycosylation pattern under physiological and pathological conditions, analysis of mucin glycans is important for understanding their specific functions during pathological conditions like cancer. Given the complexity of mucin glycans, several sophisticated analytical tools such as HPLC, mass spectrometry, and lectin sandwich assays are employed for glyco-analysis of mucins. However the specialized expertise and instrumentation required for such analysis are beyond the reach of an average cancer biology laboratory. We described in this chapter the utility of the simple electrophoresis/immunoblotting method to examine the mucin glycan epitopes, using specific antibodies and lectins.

Original languageEnglish (US)
Title of host publicationPancreatic Cancer
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages331-340
Number of pages10
ISBN (Print)9781627032865
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume980
ISSN (Print)1064-3745

Keywords

  • Lectins
  • Mucin
  • Mucin glycans
  • Mucin immunoprecipitation
  • Mucin-associated carbohydrate glycans
  • Tumor-associated carbohydrate antigen
  • Western blot/Western transfer

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Gnanapragassam, V. S., Jain, M., & Batra, S. K. (2013). Analysis of tumor-associated mucin glycotopes by western transfer methods. In Pancreatic Cancer: Methods and Protocols (pp. 331-340). (Methods in Molecular Biology; Vol. 980). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-287-2_19