Analytical methods for kinetic studies of biological interactions: A review

Xiwei Zheng, Cong Bi, Zhao Li, Maria Podariu, David S. Hage

Research output: Contribution to journalReview articlepeer-review

60 Scopus citations


The rates at which biological interactions occur can provide important information concerning the mechanism and behavior of these processes in living systems. This review discusses several analytical methods that can be used to examine the kinetics of biological interactions. These techniques include common or traditional methods such as stopped-flow analysis and surface plasmon resonance spectroscopy, as well as alternative methods based on affinity chromatography and capillary electrophoresis. The general principles and theory behind these approaches are examined, and it is shown how each technique can be utilized to provide information on the kinetics of biological interactions. Examples of applications are also given for each method. In addition, a discussion is provided on the relative advantages or potential limitations of each technique regarding its use in kinetic studies.

Original languageEnglish (US)
Pages (from-to)163-180
Number of pages18
JournalJournal of Pharmaceutical and Biomedical Analysis
StatePublished - Sep 1 2015


  • Affinity chromatography
  • Capillary electrophoresis
  • Kinetics of biological interactions
  • Stopped-flow analysis
  • Surface plasmon resonance spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry


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