TY - JOUR
T1 - Analytical methods for kinetic studies of biological interactions
T2 - A review
AU - Zheng, Xiwei
AU - Bi, Cong
AU - Li, Zhao
AU - Podariu, Maria
AU - Hage, David S.
N1 - Funding Information:
Portions of this work were supported by the NIH under grants R01 GM044931 and R01 DK069629 , the University of Nebraska UCARE program, and the NSF/EPSCoR program under grant EPS-1004094 .
Publisher Copyright:
© 2015 Elsevier B.V.
PY - 2015/9/1
Y1 - 2015/9/1
N2 - The rates at which biological interactions occur can provide important information concerning the mechanism and behavior of these processes in living systems. This review discusses several analytical methods that can be used to examine the kinetics of biological interactions. These techniques include common or traditional methods such as stopped-flow analysis and surface plasmon resonance spectroscopy, as well as alternative methods based on affinity chromatography and capillary electrophoresis. The general principles and theory behind these approaches are examined, and it is shown how each technique can be utilized to provide information on the kinetics of biological interactions. Examples of applications are also given for each method. In addition, a discussion is provided on the relative advantages or potential limitations of each technique regarding its use in kinetic studies.
AB - The rates at which biological interactions occur can provide important information concerning the mechanism and behavior of these processes in living systems. This review discusses several analytical methods that can be used to examine the kinetics of biological interactions. These techniques include common or traditional methods such as stopped-flow analysis and surface plasmon resonance spectroscopy, as well as alternative methods based on affinity chromatography and capillary electrophoresis. The general principles and theory behind these approaches are examined, and it is shown how each technique can be utilized to provide information on the kinetics of biological interactions. Examples of applications are also given for each method. In addition, a discussion is provided on the relative advantages or potential limitations of each technique regarding its use in kinetic studies.
KW - Affinity chromatography
KW - Capillary electrophoresis
KW - Kinetics of biological interactions
KW - Stopped-flow analysis
KW - Surface plasmon resonance spectroscopy
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U2 - 10.1016/j.jpba.2015.01.042
DO - 10.1016/j.jpba.2015.01.042
M3 - Review article
C2 - 25700721
AN - SCOPUS:84939255379
SN - 0731-7085
VL - 113
SP - 163
EP - 180
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
ER -