Analyzing endodontic infections by deep coverage pyrosequencing

L. Li, W. W.L. Hsiao, R. Nandakumar, S. M. Barbuto, E. F. Mongodin, B. J. Paster, C. M. Fraser-Liggett, A. F. Fouad

Research output: Contribution to journalArticle

66 Scopus citations

Abstract

Bacterial diversity in endodontic infections has not been sufficiently studied. The use of modern pyrosequencing technology should allow for more comprehensive analysis than traditional Sanger sequencing. This study investigated bacterial diversity in endodontic infections through taxonomic classification based on 16S rRNA gene sequences generated by 454 GS-FLX pyrosequencing and conventional Sanger capillary sequencing technologies. Sequencings were performed on 7 specimens from endodontic infections. On average, 47 vs. 28,590 sequences were obtained per sample for Sanger sequencing vs. pyrosequencing, representing a 600-fold difference in ĝ€depth-of- coverage". Based on Ribosomal Database Project (RDP II) Classifier analysis, pyrosequencing identified 179 bacterial genera in 13 phyla, which was significantly more than Sanger sequencing. The phylum Bacteroidetes was the most prevalent bacterial phylum. These results indicate that bacterial communities in endodontic infections are more diverse than previously demonstrated. In addition, deep-coverage pyrosequencing of the 16S rRNA gene revealed low-abundance micro-organisms with potential clinical implications.

Original languageEnglish (US)
Pages (from-to)980-984
Number of pages5
JournalJournal of Dental Research
Volume89
Issue number9
DOIs
StatePublished - Sep 2010

Keywords

  • 16S rRNA gene
  • bacterial diversity
  • endodontic infection
  • pyrosequencing
  • taxonomy

ASJC Scopus subject areas

  • Dentistry(all)

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    Li, L., Hsiao, W. W. L., Nandakumar, R., Barbuto, S. M., Mongodin, E. F., Paster, B. J., Fraser-Liggett, C. M., & Fouad, A. F. (2010). Analyzing endodontic infections by deep coverage pyrosequencing. Journal of Dental Research, 89(9), 980-984. https://doi.org/10.1177/0022034510370026