Anion modulation of calcium current voltage dependence and amplitude in salamander rods

Wallace B. Thoreson; Salvatore L. Stella

doi:10.1016/S0005-2736(99)00257-6

Anion modulation of calcium current voltage dependence and amplitude in salamander rods

Wallace B. Thoreson, Salvatore L. Stella

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Hofmeister anions were used to investigate the ability of Cl^- replacement to produce inhibition and a hyperpolarizing activation shift in L-type Ca²⁺ currents (I(Ca)) of rod photoreceptors. Inhibition of I(Ca) largely followed the Hofmeister sequence: Cl^-=Br^-<NO^-₃<I^-<ClO^-₄ (ClO^-₄ caused the greatest suppression). Anion-induced hyperpolarizing activation shifts also followed the Hofmeister sequence: Cl^-<Br^-<NO^-₃<I^-<ClO^-₄ (ClO^-₄ caused the largest shift). Agreement with the Hofmeister sequence suggests that these effects are due to anion interactions at the membrane surface. Hofmeister anions also caused similar hyperpolarizing shifts in the voltage dependence of inwardly rectifying cation currents (I(h)) and outward K⁺ currents (I(K)) consistent with the hypothesis that hyperpolarizing shifts arise from anion effects on membrane surface potential. Sulfate and phosphate inhibited rod I(Ca) and phosphate caused a significant leftward activation shift suggesting these anions are strongly adsorbed to the membrane. Because of the overlap between the physiological voltage range and the lower part of the I(Ca) activation curve, anion effects on amplitude and activation may influence synaptic transmission at the first retinal synapse. Copyright (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	142-150
Number of pages	9
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1464
Issue number	1
DOIs	https://doi.org/10.1016/S0005-2736(99)00257-6
State	Published - Mar 15 2000

Keywords

Chloride
Hofmeister anion
L-Type calcium current
Photoreceptor
Retina

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/S0005-2736(99)00257-6

Cite this

@article{e0f095db4252498191244b78697fc691,

title = "Anion modulation of calcium current voltage dependence and amplitude in salamander rods",

abstract = "Hofmeister anions were used to investigate the ability of Cl- replacement to produce inhibition and a hyperpolarizing activation shift in L-type Ca2+ currents (I(Ca)) of rod photoreceptors. Inhibition of I(Ca) largely followed the Hofmeister sequence: Cl-=Br--3--4 (ClO-4 caused the greatest suppression). Anion-induced hyperpolarizing activation shifts also followed the Hofmeister sequence: Cl---3--4 (ClO-4 caused the largest shift). Agreement with the Hofmeister sequence suggests that these effects are due to anion interactions at the membrane surface. Hofmeister anions also caused similar hyperpolarizing shifts in the voltage dependence of inwardly rectifying cation currents (I(h)) and outward K+ currents (I(K)) consistent with the hypothesis that hyperpolarizing shifts arise from anion effects on membrane surface potential. Sulfate and phosphate inhibited rod I(Ca) and phosphate caused a significant leftward activation shift suggesting these anions are strongly adsorbed to the membrane. Because of the overlap between the physiological voltage range and the lower part of the I(Ca) activation curve, anion effects on amplitude and activation may influence synaptic transmission at the first retinal synapse. Copyright (C) 2000 Elsevier Science B.V.",

keywords = "Chloride, Hofmeister anion, L-Type calcium current, Photoreceptor, Retina",

author = "Thoreson, {Wallace B.} and Stella, {Salvatore L.}",

note = "Funding Information: This work was supported by NIH grant EY-10542, the Gifford Foundation (Omaha, NE), the Nebraska Lion{\textquoteright}s Eye Research Institute, and a Research to Prevent Blindness Challenge grant to the UNMC Department of Ophthalmology. WBT is the recipient of a Research to Prevent Blindness Career Development Award. We thank Dr. R.F. Miller, University of Minnesota, for his helpful comments on the manuscript.",

year = "2000",

month = mar,

day = "15",

doi = "10.1016/S0005-2736(99)00257-6",

language = "English (US)",

volume = "1464",

pages = "142--150",

journal = "Biochimica et Biophysica Acta - Biomembranes",

issn = "0005-2736",

publisher = "Elsevier B.V.",

number = "1",

}

TY - JOUR

T1 - Anion modulation of calcium current voltage dependence and amplitude in salamander rods

AU - Thoreson, Wallace B.

AU - Stella, Salvatore L.

N1 - Funding Information: This work was supported by NIH grant EY-10542, the Gifford Foundation (Omaha, NE), the Nebraska Lion’s Eye Research Institute, and a Research to Prevent Blindness Challenge grant to the UNMC Department of Ophthalmology. WBT is the recipient of a Research to Prevent Blindness Career Development Award. We thank Dr. R.F. Miller, University of Minnesota, for his helpful comments on the manuscript.

PY - 2000/3/15

Y1 - 2000/3/15

N2 - Hofmeister anions were used to investigate the ability of Cl- replacement to produce inhibition and a hyperpolarizing activation shift in L-type Ca2+ currents (I(Ca)) of rod photoreceptors. Inhibition of I(Ca) largely followed the Hofmeister sequence: Cl-=Br--3--4 (ClO-4 caused the greatest suppression). Anion-induced hyperpolarizing activation shifts also followed the Hofmeister sequence: Cl---3--4 (ClO-4 caused the largest shift). Agreement with the Hofmeister sequence suggests that these effects are due to anion interactions at the membrane surface. Hofmeister anions also caused similar hyperpolarizing shifts in the voltage dependence of inwardly rectifying cation currents (I(h)) and outward K+ currents (I(K)) consistent with the hypothesis that hyperpolarizing shifts arise from anion effects on membrane surface potential. Sulfate and phosphate inhibited rod I(Ca) and phosphate caused a significant leftward activation shift suggesting these anions are strongly adsorbed to the membrane. Because of the overlap between the physiological voltage range and the lower part of the I(Ca) activation curve, anion effects on amplitude and activation may influence synaptic transmission at the first retinal synapse. Copyright (C) 2000 Elsevier Science B.V.

AB - Hofmeister anions were used to investigate the ability of Cl- replacement to produce inhibition and a hyperpolarizing activation shift in L-type Ca2+ currents (I(Ca)) of rod photoreceptors. Inhibition of I(Ca) largely followed the Hofmeister sequence: Cl-=Br--3--4 (ClO-4 caused the greatest suppression). Anion-induced hyperpolarizing activation shifts also followed the Hofmeister sequence: Cl---3--4 (ClO-4 caused the largest shift). Agreement with the Hofmeister sequence suggests that these effects are due to anion interactions at the membrane surface. Hofmeister anions also caused similar hyperpolarizing shifts in the voltage dependence of inwardly rectifying cation currents (I(h)) and outward K+ currents (I(K)) consistent with the hypothesis that hyperpolarizing shifts arise from anion effects on membrane surface potential. Sulfate and phosphate inhibited rod I(Ca) and phosphate caused a significant leftward activation shift suggesting these anions are strongly adsorbed to the membrane. Because of the overlap between the physiological voltage range and the lower part of the I(Ca) activation curve, anion effects on amplitude and activation may influence synaptic transmission at the first retinal synapse. Copyright (C) 2000 Elsevier Science B.V.

KW - Chloride

KW - Hofmeister anion

KW - L-Type calcium current

KW - Photoreceptor

KW - Retina

UR - http://www.scopus.com/inward/record.url?scp=0033962803&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033962803&partnerID=8YFLogxK

U2 - 10.1016/S0005-2736(99)00257-6

DO - 10.1016/S0005-2736(99)00257-6

M3 - Article

C2 - 10704928

AN - SCOPUS:0033962803

SN - 0005-2736

VL - 1464

SP - 142

EP - 150

JO - Biochimica et Biophysica Acta - Biomembranes

JF - Biochimica et Biophysica Acta - Biomembranes

IS - 1

ER -

Anion modulation of calcium current voltage dependence and amplitude in salamander rods

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this