Antagonistic effects of isofloxythepin on dopamine D1 and D2 receptors and behaviors in rats

Yuen Sum Lau, Yiu K. Fung, Todd M. Anderson

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

1. In vitro, isofloxythepin competed for the binding of [3H]SCH 23390 to striatal D1 reccptors and for the binding of [3H]spiperone to striatal D2 receptors with IC50 values of 6.1 (±1.2)x 10-10 M and 8.4 (±2.6) x 10-10 M, respectively. Isofloxythepin further inhibited the D1 receptor mediated, dopamine stimulated adenylate cyclase in the striatal tissue. 2. Fifteen hours after rats were injected with a single dose of isofloxythepin (1 mg/kg, SC), the ex vivo binding curve of [3H]spiperone to striatal D2 receptors was markedly inhibited, whereas the binding curve of [3H]SCH 23390 to striatal D1 receptors was unaffected. Fifteen hours after isofloxythepin pretreatment, D1 agonist SKF 38393 (15 mg/kg, IP)-stimulated grooming behavior was not affected, whereas the D2 agonist quinpirole (3 mg/kg, IP) stimulated stereotyped behavior was completely abolished. 4. On the basis of the findings from in vivo studies, we conclude that, although isofloxythepin is found to have high affinity for both D1 and D2 receptors in vitro, it lacks D1 antagonistic potency in vivo.

Original languageEnglish (US)
Pages (from-to)729-736
Number of pages8
JournalGeneral Pharmacology
Volume29
Issue number5
DOIs
StatePublished - Nov 1997

Keywords

  • D and D receptors
  • Grooming behavior
  • Isofloxythepin
  • Nucleus accumbens
  • Stereotyped behavior
  • Striatum

ASJC Scopus subject areas

  • Pharmacology

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