Antiapoptotic Activity of PAI-2 is Affected by Its Cellular Localization

Yu Qing Zhang, Min Hou, Xia Wang, Xing Hui Sun, Ping Li, Yun Song Zhu

Research output: Contribution to journalArticle

Abstract

Purpose: To study the effect of cellular location of PAI-2 on its antiapoptotic activity. Methods: The cDNA of PAI-2-NLS mutant, 3′-terminus of which a nuclear localization sequence was inserted downstream, was cloned in-frame into eukaryotic expression vector, pEGFPC3, producing pEGFPC3-PAI2-NLS. After transfected into HeLa cells, different distributions of PAI-2 and PAI2-NLS mutant in HeLa cells were observed through fluorescence microscopy, and MTT assay was used to analyze the antiapoptotic activity of this PAI-2 mutant. Results: Due to the nuclear localization sequence, almost all PAI-2 mutants entered nuclei, while wide type PAI-2 distributed evenly in the cytoplasm and nucleus. The results of MTT assay indicated that if PAI-2 was located in the nuclei, it would lose its antiapoptotic activity against TNF-α. Conclusions: All the results demonstrate that antiapoptotic activity of PAI-2 is affected by its cellular localization. In addition, the treatment of TNF-α would not affect the distribution of PAI-2 in HeLa cells.

Original languageEnglish (US)
Pages (from-to)531-533+536
JournalFudan University Journal of Medical Sciences
Volume30
Issue number6
StatePublished - Nov 2003

Keywords

  • Apoptosis
  • Nuclear localization sequence
  • Plasminogen activator inhibitor type-2

ASJC Scopus subject areas

  • Medicine(all)

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