Abstract
Purpose: To study the effect of cellular location of PAI-2 on its antiapoptotic activity. Methods: The cDNA of PAI-2-NLS mutant, 3′-terminus of which a nuclear localization sequence was inserted downstream, was cloned in-frame into eukaryotic expression vector, pEGFPC3, producing pEGFPC3-PAI2-NLS. After transfected into HeLa cells, different distributions of PAI-2 and PAI2-NLS mutant in HeLa cells were observed through fluorescence microscopy, and MTT assay was used to analyze the antiapoptotic activity of this PAI-2 mutant. Results: Due to the nuclear localization sequence, almost all PAI-2 mutants entered nuclei, while wide type PAI-2 distributed evenly in the cytoplasm and nucleus. The results of MTT assay indicated that if PAI-2 was located in the nuclei, it would lose its antiapoptotic activity against TNF-α. Conclusions: All the results demonstrate that antiapoptotic activity of PAI-2 is affected by its cellular localization. In addition, the treatment of TNF-α would not affect the distribution of PAI-2 in HeLa cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 531-533+536 |
| Journal | Fudan University Journal of Medical Sciences |
| Volume | 30 |
| Issue number | 6 |
| State | Published - Nov 2003 |
| Externally published | Yes |
Keywords
- Apoptosis
- Nuclear localization sequence
- Plasminogen activator inhibitor type-2
ASJC Scopus subject areas
- Medicine(all)