Ag nanoparticles were synthesized in pure water by a nanosecond pulsed laser ablation process and characterized using transmission electron microscopy and ultraviolet-visible spectroscopy. The as-synthesized nanoparticles were determined to have an average particle size of 10.17 ± 2.95 nm. Their absorption spectra were consistent with that of Ag nanoparticles produced through chemical methods. The antibacterial properties of Ag nanoparticles at concentrations of 100 and 250 μg/ml were studied against the gram-positive bacterium Staphylococcus aureus at two infectious inocula (103 and 105 CFU/ml). Over a 24-h test period, the samples containing 100 μg/ml exhibited early bacteriostatic activity but did not stop long-term bacterial growth, while the 250 μg/ml samples demonstrated bactericidal and bacteriostatic effects. These results highlight the use of Ag nanoparticles synthesized through laser ablation as green and effective antibacterial agents for biomedical applications.
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering