TY - JOUR
T1 - Antibodies against malondialdehyde-acetaldehyde adducts can help identify patients with abdominal aortic aneurysm
AU - Carson, Jeffrey S.
AU - Xiong, Wanfen
AU - Dale, Matthew
AU - Yu, Fang
AU - Duryee, Michael J.
AU - Anderson, Daniel R
AU - Thiele, Geoffrey Milton
AU - Baxter, Bernard Timothy
N1 - Funding Information:
The research reported in this manuscript was supported by National Institutes of Health grants number 5R01HL062400-12 and 5R01AG037120 (B.T.B), American Heart Association grant number 12GRNT12190022 (G.M.T), and the University of Nebraska Medical Center Departments of Surgery and Internal Medicine .
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Objective Abdominal aortic aneurysm (AAA) is a pathologic dilation of the aorta. Inflammation of the aortic wall has been shown to be involved in AAA formation. Malondialdehyde-acetaldehyde (MAA) adducts are MAA/protein hybrids with immunogenic, proinflammatory, and profibrotic properties. Levels of MAA adducts are elevated in patients with coronary artery disease; however, the role of MAA adducts in AAA is unclear. We hypothesize that levels of circulating antibodies against MAA adducts are increased in patients with AAA. Methods Plasma samples were collected from mice and patients with AAA and control patients with atherosclerosis but not AAA. AAA was induced in mice by a standard CaCl2 protocol, with matching sham mice. Plasma levels of anti-MAA antibodies were quantified by enzyme-linked immunosorbent assay. Results Patients with AAA exhibited higher levels of immunoglobulin G and immunoglobulin A anti-MAA antibody subtypes (P =.049 and.026, respectively) compared with control patients. Conversely, immunoglobulin M anti-MAA antibodies in AAA patients were lower compared with control patients (P =.018). In CaCl2-treated mice, immunoglobulin G anti-MAA antibodies were elevated after AAA formation (P =.006). Conclusions The pattern of anti-MAA antibodies is able to distinguish between patients with AAA and patients with atherosclerosis but no AAA. These results demonstrate that MAA adducts are associated with AAA and suggest that they may play a role in either initiating or propagating chronic inflammation in AAA.
AB - Objective Abdominal aortic aneurysm (AAA) is a pathologic dilation of the aorta. Inflammation of the aortic wall has been shown to be involved in AAA formation. Malondialdehyde-acetaldehyde (MAA) adducts are MAA/protein hybrids with immunogenic, proinflammatory, and profibrotic properties. Levels of MAA adducts are elevated in patients with coronary artery disease; however, the role of MAA adducts in AAA is unclear. We hypothesize that levels of circulating antibodies against MAA adducts are increased in patients with AAA. Methods Plasma samples were collected from mice and patients with AAA and control patients with atherosclerosis but not AAA. AAA was induced in mice by a standard CaCl2 protocol, with matching sham mice. Plasma levels of anti-MAA antibodies were quantified by enzyme-linked immunosorbent assay. Results Patients with AAA exhibited higher levels of immunoglobulin G and immunoglobulin A anti-MAA antibody subtypes (P =.049 and.026, respectively) compared with control patients. Conversely, immunoglobulin M anti-MAA antibodies in AAA patients were lower compared with control patients (P =.018). In CaCl2-treated mice, immunoglobulin G anti-MAA antibodies were elevated after AAA formation (P =.006). Conclusions The pattern of anti-MAA antibodies is able to distinguish between patients with AAA and patients with atherosclerosis but no AAA. These results demonstrate that MAA adducts are associated with AAA and suggest that they may play a role in either initiating or propagating chronic inflammation in AAA.
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U2 - 10.1016/j.jvs.2014.08.117
DO - 10.1016/j.jvs.2014.08.117
M3 - Article
C2 - 25724613
AN - SCOPUS:84957430628
SN - 0741-5214
VL - 63
SP - 477
EP - 485
JO - Journal of Vascular Surgery
JF - Journal of Vascular Surgery
IS - 2
ER -