TY - JOUR
T1 - Antibody cross-linking of the thymocyte-specific cell surface molecule CTX causes abnormal mitosis and multinucleation of tumor cells
AU - Robert, Jacques
AU - Brown, Deborah M.
AU - Pasquier, Louis Du
AU - Cohen, Nicholas
N1 - Funding Information:
We gratefully acknowledge Chantal Guiet and Janice Miller for their technical participation in this work, Andre Traunecker for his image analysis of video recording, and Dr. Max Cooper for kindly providing us with Xenopus-speci®c anti-CD5 mAb 2B1. We also thank Edith Lord for her criticisms and helpful discussions. Research at the University of Rochester was supported by Grant USPHS R37 HD-07901. The Basel Institute for Immunology was founded and is supported by F. Hoffman La Roche, Basel, Switzerland.
PY - 1997/8/25
Y1 - 1997/8/25
N2 - The thymocyte-specific cell surface molecule CTX is a developmentally regulated type I transmembrane protein of the immunoglobulin superfamily which, in the amphibian Xenopus, is exclusively expressed by a large fraction of cortical thymocytes and by different cell lines derived from independent spontaneous thymic tumors. Antibody cross-linking of CTX in vitro inhibits the growth of tumor cells and causes morphological alterations. Cells divide abnormally, accumulate in the G2/M phase of the cell cycle, and become multi- nucleated. This demonstrates, for the fast time, that multinucleation can be induced by specifically cross-linking a cell surface molecule.
AB - The thymocyte-specific cell surface molecule CTX is a developmentally regulated type I transmembrane protein of the immunoglobulin superfamily which, in the amphibian Xenopus, is exclusively expressed by a large fraction of cortical thymocytes and by different cell lines derived from independent spontaneous thymic tumors. Antibody cross-linking of CTX in vitro inhibits the growth of tumor cells and causes morphological alterations. Cells divide abnormally, accumulate in the G2/M phase of the cell cycle, and become multi- nucleated. This demonstrates, for the fast time, that multinucleation can be induced by specifically cross-linking a cell surface molecule.
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U2 - 10.1006/excr.1997.3672
DO - 10.1006/excr.1997.3672
M3 - Article
C2 - 9281372
AN - SCOPUS:0031586363
SN - 0014-4827
VL - 235
SP - 227
EP - 237
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 1
ER -