Antibody labeling with radioiodine and radiometals

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Antibodies have been conjugated to radionuclides for various in vitro and in vivo applications. Radiolabeled antibodies have been used in clinics and research for diagnostic applications both in vitro as reagents in bioassays and in vivo as imaging agents. Further, radiolabeled antibodies are used as direct therapeutic agents for cancer radioimmunotherapy or as tracers for studying the pharmacokinetics and biodistribution of therapeutic antibodies. Antibodies are labeled with radiohalogens or radiometals, and the choice of candidate radionuclides for a given application is dictated by their emission range and half-life. The conjugation chemistry for the coupling of MAbs with the radiometals requires a chelator, whereas radiohalogens can be incorporated directly in the antibody backbone. In this chapter, we describe the commonly used methods for radiolabeling and characterizing the antibodies most commonly used radiohalogens (125I/131I) and radiometals (177Lu/99mTc).

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages147-157
Number of pages11
Volume1141
ISBN (Print)9781493903627
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1141
ISSN (Print)10643745

Keywords

  • 177Lu
  • 99mTc
  • Antibodies
  • Chelator
  • DOTA
  • DTPA
  • Iodogen
  • Radioiodine
  • Radiolabeling
  • Radiometals
  • Tracers

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Gupta, S., Batra, S. K., & Jain, M. (2014). Antibody labeling with radioiodine and radiometals. In Methods in Molecular Biology (Vol. 1141, pp. 147-157). (Methods in Molecular Biology; Vol. 1141). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-363-4_9