ApoB-independent enzyme immunoassay for lipoprotein(a) by capture on immobilized lectin (Jacalin)

Anuradha Sreekumar, Geetha Mandagini, Sabarinath P. Subramanian, Appukuttan P. Sankunni

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 ± 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV = 6.4%).

Original languageEnglish (US)
Pages (from-to)166-179
Number of pages14
JournalJournal of Immunoassay and Immunochemistry
Issue number2
StatePublished - Apr 1 2013
Externally publishedYes


  • enzyme-linked immunoassay
  • jacalin
  • lipoprotein(a)

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Medical Laboratory Technology


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