ApoB-independent enzyme immunoassay for lipoprotein(a) by capture on immobilized lectin (Jacalin)

Anuradha Sreekumar; Geetha Mandagini; Sabarinath P. Subramanian; Appukuttan P. Sankunni

doi:10.1080/15321819.2012.699493

ApoB-independent enzyme immunoassay for lipoprotein(a) by capture on immobilized lectin (Jacalin)

Anuradha Sreekumar, Geetha Mandagini, Sabarinath P. Subramanian, Appukuttan P. Sankunni

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 ± 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV = 6.4%).

Original language	English (US)
Pages (from-to)	166-179
Number of pages	14
Journal	Journal of Immunoassay and Immunochemistry
Volume	34
Issue number	2
DOIs	https://doi.org/10.1080/15321819.2012.699493
State	Published - Apr 1 2013
Externally published	Yes

Keywords

enzyme-linked immunoassay
jacalin
lipoprotein(a)

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Clinical Biochemistry
Medical Laboratory Technology

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title = "ApoB-independent enzyme immunoassay for lipoprotein(a) by capture on immobilized lectin (Jacalin)",

abstract = "Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 ± 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV = 6.4%).",

keywords = "enzyme-linked immunoassay, jacalin, lipoprotein(a)",

author = "Anuradha Sreekumar and Geetha Mandagini and Subramanian, {Sabarinath P.} and Sankunni, {Appukuttan P.}",

note = "Funding Information: A fellowship to Ms. Anuradha Sreekumar was received from the Council of Scientific and Industrial research, Government of India. The authors are grateful to the Division of Blood Transfusion Services of this institute for gift of serum and outdated plasma samples.",

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doi = "10.1080/15321819.2012.699493",

language = "English (US)",

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T1 - ApoB-independent enzyme immunoassay for lipoprotein(a) by capture on immobilized lectin (Jacalin)

AU - Sreekumar, Anuradha

AU - Mandagini, Geetha

AU - Subramanian, Sabarinath P.

AU - Sankunni, Appukuttan P.

N1 - Funding Information: A fellowship to Ms. Anuradha Sreekumar was received from the Council of Scientific and Industrial research, Government of India. The authors are grateful to the Division of Blood Transfusion Services of this institute for gift of serum and outdated plasma samples.

PY - 2013/4/1

Y1 - 2013/4/1

N2 - Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 ± 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV = 6.4%).

AB - Enzyme immunoassay for lipoprotein(a) [Lp(a)] using antibodies to both apoB and apo(a) subunits (a-B assay) is shown to be affected by differential masking of apoB by apo(a) and the presence of LDL-Lp(a) adducts. An apoB-independent immunoassay by capturing Lp(a) through its O-glycans on microplate-coated lectin jacalin and quantitation using peroxidase-labeled anti-apo(a) (J-a assay) is described. J-a assay response is linear, more than twice as sensitive as a-B assay, and is suppressed only 18 ± 5% by non-Lp(a) O-glycan-containing proteins of serum. Wide variations in IgA did not significantly affect Lp(a) binding to jacalin (CV = 6.4%).

KW - enzyme-linked immunoassay

KW - jacalin

KW - lipoprotein(a)

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DO - 10.1080/15321819.2012.699493

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SN - 1532-1819

VL - 34

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JO - Journal of Immunoassay and Immunochemistry

JF - Journal of Immunoassay and Immunochemistry

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ER -

ApoB-independent enzyme immunoassay for lipoprotein(a) by capture on immobilized lectin (Jacalin)

Abstract

Keywords

ASJC Scopus subject areas

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