Application of design-of-experiments procedures to optimize efficiently pretreatment of lipase for use in a nonaqueous reaction

Rafael A. Garcia, Mark R. Riley

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A variety of different pretreatments can improve the performance of enzymes in nonpolar reaction media. These pretreatments have primarily been studied in isolation; however, interactions between some pairs of pretreatments are known to exist. The presence of these interactions complicates the design of an optimum multifactor pretreatment. Modern design-of-experiments techniques allow the simultaneous optimization of two or more variables. To improve the performance of lipase in a model reaction, we used a technique called the method of steepest ascent to optimize three variables simultaneously: pretreatment pH and sodium phosphate concentration, and the concentration of acetic acid (one of the reactants) in the reaction mixture. In only 26 experimental runs, this optimization process determined a combination of variable settings that yielded a reaction product approx 180 times faster than achieved with untreated enzyme. Evidence is presented to demonstrate that locating this optimum with single-factor experiments would be inefficient. This article demonstrates the efficiency of the method of steepest ascent particularly for evaluation of enzymatic reaction conditions exhibiting significant interactions.

Original languageEnglish (US)
Pages (from-to)69-78
Number of pages10
JournalApplied Biochemistry and Biotechnology
Volume127
Issue number2
DOIs
StatePublished - Nov 2005

Keywords

  • Esterification
  • Fragrance
  • Lipase
  • Lyophilization
  • Nonaqueous enzymology
  • Optimization
  • Pretreatment

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Application of design-of-experiments procedures to optimize efficiently pretreatment of lipase for use in a nonaqueous reaction'. Together they form a unique fingerprint.

  • Cite this